LOCUS       sequence-49289-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     MLV retroviral system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBS-CMV-gagpol
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             147..563
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     493..515
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     531..547
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
       61 ccgatccagc ctccgcggcc ccaagcttgg gcatcgttct gtgttgtctc tgtctgactg
      121 tgtttctgta tttgtctgaa aatatgggcc agactgttac cactccctta agtttgacct
      181 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
      241 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
      301 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
      361 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
      421 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
      481 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
      541 ccctcactcc ttctctaggc gccaaaccta aacctcaagt tctttctgac agtggggggc
      601 cgctcatcga cctacttaca gaagaccccc cgccttatag ggacccaaga ccaccccctt
      661 ccgacaggga cggaaatggt ggagaagcga cccctgcggg agaggcaccg gacccctccc
      721 caatggcatc tcgcctacgt gggagacggg agccccctgt ggccgactcc actacctcg
//
