LOCUS       sequence-49290-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Markus SM, Punch JJ, Lee WL
  TITLE     Motor- and tail-dependent targeting of dynein to microtubule plus 
            ends and the cell cortex.
  JOURNAL   Curr Biol. 2009 Feb 10;19(3):196-205. Epub 2009 Jan 29.
  PUBMED    19185494
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2009-02-10"; volume: "19(3)"; pages: "196-205. Epub 
            2009 Jan 29"
COMMENT     Sequence Label: pRS304:3mCherry
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             198..908
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(343..361)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     611..630
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(910..926)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(910..926)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
       61 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      121 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      181 acaagactag gagatccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
      241 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      301 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      361 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      421 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      481 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      541 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      601 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      661 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
      721 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      781 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      841 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      901 acaagtagag atccactagt tctagagcgg ccgccaccgc ggtggagctc caattcgccc
      961 tatagtagt
//
