LOCUS       sequence-49292-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Markus SM, Punch JJ, Lee WL
  TITLE     Motor- and tail-dependent targeting of dynein to microtubule plus 
            ends and the cell cortex.
  JOURNAL   Curr Biol. 2009 Feb 10;19(3):196-205. Epub 2009 Jan 29.
  PUBMED    19185494
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2009-02-10"; volume: "19(3)"; pages: "196-205. Epub 
            2009 Jan 29"
COMMENT     Sequence Label: pRS304:3mCherry
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     64..80
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     65..81
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             132..839
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(277..295)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     545..564
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 attacgccaa gctcggaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
       61 ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatccg
      121 ctccaagtgg aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      181 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      241 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      301 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      361 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      421 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      481 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      541 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
      601 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
      661 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      721 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      781 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagc
      841 ctaggatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      901 tcaaggtgca catggagggc tccgtgaacg
//
