LOCUS       sequence-492944-        2697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMB1o1_Blue
FEATURES             Location/Qualifiers
     source          1..2697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..69
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        123..141
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     123..140
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    148..204
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(206..225)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(207..225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(232..249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(232..248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(241..263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        547..618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(619..687,688..1479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(837..856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1650..2238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2139..2158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2392..2409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2424..2564
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 ggtctcagga gagagacgcc tgttgacaat taatcatcgg catagtatat cggcatagta
       61 taatacgaca aggtgaggaa ctaaacccag gaggcagatc atgaccatga ttacgccaag
      121 ctatttaggt gacactatag aatactcaag cttgcatgcc tgcaggtcga ctctagagga
      181 tccccgggta ccgagctcga attcgcccta tagtgagtcg tattacaatt cactggccgt
      241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      361 acagttgcgc agcctgaatg gcgaatgacg cctgatgcgg tattttctcc ttacgcatct
      421 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
      481 gcgtctctcg ctgtcatgag accggatctt gacacggtta tccacagaat caggggatac
      541 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      601 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      661 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      721 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      781 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      841 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      901 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      961 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1021 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1081 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1141 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     1201 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1261 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1321 gccggtgagc gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1381 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1441 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     1801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2281 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2341 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2401 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2461 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2521 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     2581 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     2641 tgaccgtttt aacgcgaatt ttaacaaaat attaacgctt acaattttaa cggatcc
//
