LOCUS       sequence-49295-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Markus SM, Plevock KM, St Germain BJ, Punch JJ, Meaden CW, Lee WL
  TITLE     Quantitative analysis of Pac1/LIS1-mediated dynein targeting: 
            Implications for regulation of dynein activity in budding yeast.
  JOURNAL   Cytoskeleton (Hoboken). 2011 Mar;68(3):157-74. doi: 
            10.1002/cm.20502. Epub 2011 Feb 3.
  PUBMED    21294277
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.20502"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2011-03"; volume: 
            "68"; issue: "3"; pages: "157-74"
COMMENT     Sequence Label: pRS303:VC
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     315..332
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    449..470
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(485..490,491..508,509..515)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    523..539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     528..550
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     547..563
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     547..563
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        584..602
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcgaggaagc gcagttcgta ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
       61 gggggaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      121 gatttttgtg atgctcgtca ggggggccga gcctatggaa aaacgccagc aacgcggcct
      181 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      241 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      301 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
      361 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      421 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      481 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      541 ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac cctcactaaa
      601 gggaacaaaa gctgggtacc gggccccccc tcgaggcttg taaaattcca aatgaattaa
      661 aaggtaaagt tatgaatcat atggacaaac aaaagaatgg tatcaaagct aacttcaaaa
      721 ttagacacaa cattgaagat ggtggtgttc aattagctga ccattatcaa caaaatactc
      781 caattggtga tggtccagtc ttgttaccag acaaccatta cttatcctat caatctgcct
      841 tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg
      901 ctggtattac ccatggtatg gatgaattgt acaaataagc ggccgccacc gcggtggagc
      961 tccaattcgc cc
//
