LOCUS       sequence-49297-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Markus SM, Plevock KM, St Germain BJ, Punch JJ, Meaden CW, Lee WL
  TITLE     Quantitative analysis of Pac1/LIS1-mediated dynein targeting: 
            Implications for regulation of dynein activity in budding yeast.
  JOURNAL   Cytoskeleton (Hoboken). 2011 Mar;68(3):157-74. doi: 
            10.1002/cm.20502. Epub 2011 Feb 3.
  PUBMED    21294277
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.20502"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2011-03"; volume: 
            "68"; issue: "3"; pages: "157-74"
COMMENT     Sequence Label: pRS303:VC
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..32
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(399..418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(400..418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(425..442)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(425..441)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(434..456)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(669..688)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcgccagct cgaaattaac cctcactaaa gggaacaaaa gctggtaccg ggccccccct
       61 cgaggcttgt aaaattccaa atgaattaaa aggtaaagtt atgaatcata tggacaaaca
      121 aaagaatggt atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca
      181 attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga
      241 caaccattac ttatcctatc aatctgcctt atccaaagat ccaaacgaaa agagagacca
      301 catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta
      361 caaataagcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag tcgtattaca
      421 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      481 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      541 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
      601 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      661 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      721 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      781 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      841 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt ccaactggac
      901 acctcact
//
