LOCUS       sequence-49299-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Tang X, Germain BS, Lee WL
  TITLE     A novel patch assembly domain in Num1 mediates dynein anchoring at 
            the cortex during spindle positioning.
  JOURNAL   J Cell Biol. 2012 Mar 19;196(6):743-56.
  PUBMED    22431751
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2012-03-19"; volume: "196(6)"; pages: "743-56"
COMMENT     Sequence Label: pBSG01
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      319..328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     436..459
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             469..489
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             496..519
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             520..543
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             554..577
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             578..622
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             629..649
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             686..859
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
ORIGIN
        1 gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg
       61 tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac cttcaagggt
      121 caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac
      181 aaagagctgg cgaaagagtt cctcgaaaac tatctgctga ctgatgaagg tctggaagcg
      241 gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga agagttggcg
      301 aaagatccac gtattgccgc caccatggaa aacgcccaga aaggtgaaat catgccgaac
      361 atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc
      421 ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctggatccga aaatttatat
      481 tttcaaggtg agctccacca ccaccaccac caccaccacc tggaagttct gttccagggg
      541 cccgggcggc cgcttggaag ttttgtttca aggtccaaaa gaaaccgctg ctgctaaatt
      601 cgaacgccag cacatggaca gcattaacga gaatctttat tttcagggcg ccgccaaatt
      661 cggccttgcg caacacgatg aagccgtgga caacaaattc aacaaagaac aacaaaacgc
      721 gttctatgag atcttacatt tacctaactt aaacgaagaa caacgaaacg ccttcatcca
      781 aagtttaaaa gatgacccaa gccaaagcgc taacctttta gcagaagcta aaaagctaaa
      841 tgatgctcag gcgccgaaag tagacgcgaa ttaagg
//
