LOCUS       sequence-49302-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Zhu Y, Xu J, Heinemann SF
  TITLE     Two pathways of synaptic vesicle retrieval revealed by 
            single-vesicle imaging.
  JOURNAL   Neuron. 2009 Feb 12;61(3):397-411.
  PUBMED    19217377
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2009-02-12"; volume: "61(3)"; pages: "397-411"
COMMENT     Sequence Label: pcDNA3-SypHluorin 1x (S1x)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      656..665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(695..723)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacgggccct ctagactcga gctcaagctt gccaccatgg acgtggtgaa
      121 tcagctggtg gctgggggtc agttccgggt ggtcaaggag ccccttggct tcgtgaaggt
      181 gctgcagtgg gtctttgcca tcttcgcctt tgctacgtgc ggcagctaca ccggagagct
      241 tcggctgagc gtggagtgtg ccaacaagac ggagagtgcc ctcaacatcg aagtcgaatt
      301 tgagtaccca ttcaggctgc accaagtgta ctttgatgca ccctcctgcg ttaaaggggg
      361 cactaccaag atcttcctag ttggtgacta ctcctcctcg gctgaattct ttgtcaccgt
      421 ggctgtgttt gccttcctct actccatggg ggccctggcc acctacatct tcctgcagaa
      481 caagtaccga gagaacaaca aagggccaat gatggacttc ctggccacag cagtgttcgc
      541 tttcatgtgg ctagttagct catccgcctg ggccaaaggc ctgtccgatg tgaagatggc
      601 cactgaccca gagaacatta tcaaggagat gcctatgtgc cgccagacag atctagccac
      661 catgggaagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      721 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      781 cggaaa
//
