LOCUS       sequence-49303-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Zhu Y, Xu J, Heinemann SF
  TITLE     Two pathways of synaptic vesicle retrieval revealed by 
            single-vesicle imaging.
  JOURNAL   Neuron. 2009 Feb 12;61(3):397-411.
  PUBMED    19217377
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2009-02-12"; volume: "61(3)"; pages: "397-411"
COMMENT     Sequence Label: pcDNA3-SypHluorin 2x (S2x)
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(44..73)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(88..114)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(578..598)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtttaactca atgatgatga tgatgatggt cgacggcgct attcagatcc tcttctgaga
       61 tgagtttttg ttctacgtaa gctatcaggc gtaatcgggc acatcgtaag ggtaggcagc
      121 ttggtaccga agcttcggat ccgacatctg attggagaag gaggtgggcg caccctgttg
      181 gccgtagcct tgctgcccat agtcgccctg aggcccgtag ccaccgccac cgccgctggc
      241 tggctgcccg taatcgggtt gataaccacc ctgaggcccg taggagtcct ggggcccata
      301 gcctccgggg ccctgcccat agcccgcatc gccgtaggca tcgccaggag ctggttgctt
      361 ttctggggcg cctggaggtg cgcgcatgaa tggggcggcc cagcctgtct ccttgaacac
      421 gaaccatagg ttgccaaccc agagcaccag gttcaggaag ccaaacacca ccgaggtgtt
      481 gagtcctgaa gtcacagggt ccctcagttc cttgcatgtg attcgagctc caccggtttt
      541 gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat
      601 gtggtctctc ttttcgttgg gatctttcga aagagtagaa gttgtaaaca ggtaatggtt
      661 gtctggtaaa aggacgggcc catcgccaat tggagtattt tgttgataat ggtctgctag
      721 ttgaacgcct ccatcttcaa tgttgtgtct aattttgaag ttagctttga ttccattctt
      781 ttgtttgtct gccatgatgt acacctggtg atcgttatag ttgtattcca atttgtgtcc
      841 aagaatgttt ccatc
//
