LOCUS       sequence-493033-       11739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acyclovir-regulated puromycin n-acetyltransferase-P2A-EGFP fusion 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Hou Q, Oleynikov M, Mei X, Dong L, Hagen T, Jaffrey SR
  TITLE     A portable poison exon for small-molecule control of mammalian gene 
            expression.
  JOURNAL   Nat Methods. 2025 Nov 3. doi: 10.1038/s41592-025-02860-7.
  PUBMED    41184552
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2025 Nov 3. doi: 10.1038/s41592-025-02860-7."
COMMENT     Sequence Label: LentiGuide Cyclone-Puro P2A EGFP
FEATURES             Location/Qualifiers
     source          1..11739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     414..434
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             524..580
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(581..583,584..1297)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(581..583,584..1294)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(623..644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(884..903)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1231..1252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1313..1901
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1366..1386)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1784..1795)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1973..2206
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2284..2405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2321..2340)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2375..2394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2445..2580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2600..2619)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2601..2619)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2629..2646)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2629..2645)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2638..2660)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2787..3242
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2874..2893)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3084..3105
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3268..3372
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3373..3441,3442..4233)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3591..3610)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4404..4992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4893..4912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5146..5163
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5280..5301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5316..5321,5322..5339,5340..5346)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5354..5370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5359..5381
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5378..5394
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5378..5394
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5413..5433
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5415..5433
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5461..5687
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5688..5868
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5915..6040
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6533..6766
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6951..6995
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7173..7421
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7173..7193
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7344..7363
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9303..9378
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9434..9551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9690..10868
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9921..10859
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10816..10836
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(10881..10900)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 acaatcaata ttaaagaatt ttgatgccaa aactattaga taaaaggtta atctacatcc
       61 ctactagaat tctcatactt aactggttgg ttatgtggaa gaaacatact ttcacaataa
      121 agagctttag gatatgatgc cattttatat cactagtagg cagaccagca gacttttttt
      181 tattgtgata tgggataacc taggcatact gcactgtaca ctctgacata tgaagtgctc
      241 tagtcaagtt taactggtgt ccacagagga catggtttaa ctggaattcg tcaagcctct
      301 ggttctaatt tctcatttgc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
      361 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
      421 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
      481 gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcc ggcgcaacaa acttctctct
      541 gctgaaacaa gccggagatg tcgaagagaa tcctggaccg gtgagcaagg gcgaggagct
      601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      661 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      781 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      841 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1021 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1081 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1261 cgggatcact ctcggcatgg acgagctgta caagtaaacg cgttaagtcg acaatcaacc
     1321 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     1381 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     1441 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     1501 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     1561 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     1621 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     1681 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     1741 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     1801 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     1861 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     1921 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     1981 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     2041 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2101 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2161 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     2221 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     2281 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2341 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2401 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     2461 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2521 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2581 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     2641 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2701 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2761 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     2821 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2881 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2941 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3001 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3061 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3121 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3181 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     3241 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3301 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3361 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3421 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3481 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3541 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3601 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3661 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3721 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3781 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3841 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3901 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3961 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4021 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4081 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4141 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4201 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4261 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4321 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4381 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4441 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4501 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4561 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4621 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4681 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4741 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4801 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4861 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4921 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4981 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5041 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5101 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5161 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5221 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     5281 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     5341 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     5401 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     5461 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     5521 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     5581 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     5641 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     5701 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5761 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5821 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     5881 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     5941 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     6001 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     6061 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     6121 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     6181 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     6241 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     6301 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     6361 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     6421 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     6481 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     6541 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     6601 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     6661 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     6721 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     6781 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     6841 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     6901 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     6961 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7021 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     7081 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     7141 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     7201 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     7261 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     7321 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     7381 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagacggt tgtaaatgag
     7441 cacacaaaat acacatgcta aaatattata ttctatgacc tttataaaat caaccaaaat
     7501 cttcttttta ataactttag tatcaataat tagaattttt atgttccttt ttgcaaactt
     7561 ttaataaaaa tgagcaaaat aaaaaaacgc tagttttagt aactcgcgtt gttttcttca
     7621 cctttaataa tagctactcc accacttgtt cctaagcggt cagctcctgc ttcaatcatt
     7681 ttttgagcat cttcaaatgt tctaactcca ccagctgctt taactaaagc attgtcttta
     7741 acaactgact tcattagttt aacatcttca aatgttgcac ctgattttga aaatcctgtt
     7801 gatgttttaa caaattctaa tccagcttca acagctattt cacaagcttt catgatttct
     7861 tcttttgtta ataaacaatt ttccataata catttaacaa catgtgatcc agctgctttt
     7921 tttacagctt tcatgtcttc taaaactaat tcataatttt tgtcttttaa tgcaccaata
     7981 tttaatacca tatcaatttc tgttgcacca tctttaattg cttcagaaac ttcgaatgct
     8041 tttgtagctg ttgtgcatgc acctagagga aaacctacaa catttgttat tcctacattt
     8101 gtgcctttta ataattcttt acaatagctt gttcaatatg aattaacaca aactgttgca
     8161 aaatcaaatt caattgcttc atcacataat tgtttaattt cagctttcgt agcatcttgt
     8221 tttaataatg tgtgatctat atatttgttt agtttcattt tttctcctat atattcattt
     8281 ttaattttaa ttctttaata atttcgtcta ctttaacttt agcgttttga acagattcac
     8341 caacacctat aaaataaatt tttagtttag gttcagttcc acttgggcga acagcaaatc
     8401 atgacttatc ttctaaataa aattttagta agtcttgtcc tggcatatta tacattccat
     8461 cgatgtagtc ttcaacatta acaactttaa gtccagcaat ttgagttaag ggtgttgctc
     8521 tcaatgattt cattaatggt tcaattttta atttcttttc ttctggttta aaattcaagt
     8581 ttaaagtgaa agtgtaatat gcacccattt ctttaaataa atcttctaaa tagtctacta
     8641 atgttttatt ttgtttttta taaaatcaag cagcctctgc tattaatata gaagcttgta
     8701 ttccatcttt atctctagct gagtcatcaa ttacatatcc ataactttct tcataagcaa
     8761 aaacaaaatt taatccgtta tcttcttctt tagcaatttc tctacccatt catttaaatc
     8821 cagttaaagt ttttacaata ttaactccat atttttcatg agcgattcta tcacccaaat
     8881 cacttgttac aaaacttgaa tatagagccg gattttttgg aatgctattt aagcgtttta
     8941 gatttgataa ttttcaatca attaaaattg gtcctgtttg atttccatct aatcttacaa
     9001 aatgaccatc atgttttatt gccattccaa atctgtcagc atctgggtca ttcataataa
     9061 taatatctgc atcatgttta ataccatatt caagcggtat ttttcatgca ggatcaaatt
     9121 ctggatttgg atttacaaca tttttaaatg tttcatcttc aaatgcatgc tcttcaacct
     9181 caataacgtt atatcctgat tcacgtaata tttttggggt aaatttagtt cctgttccat
     9241 taactgcgct aaaaataatt tttaaatctt ttttagcttc ttgctctttt ttgtacgtct
     9301 ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     9361 gtggcaccga gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga caaatggcag
     9421 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     9481 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     9541 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     9601 gggcccgggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     9661 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     9721 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
     9781 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     9841 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     9901 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     9961 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
    10021 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
    10081 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
    10141 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
    10201 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
    10261 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
    10321 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
    10381 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
    10441 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
    10501 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
    10561 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
    10621 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
    10681 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
    10741 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
    10801 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
    10861 tgtcgtgacg tacggccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
    10921 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
    10981 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
    11041 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg
    11101 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
    11161 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
    11221 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc
    11281 accaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt
    11341 cgagacagag aagactcttg cgtttctgat aggcacctat tggtctgatc atattttgtt
    11401 gaataaaata agtaaaatgt cttgtgaaac aaaatgcttt ttaacatcca tataaagcta
    11461 tctatatata gctatctatg tctatatagc tatttttttt aacttccttt attttcctta
    11521 cagggtttca gacaaaatca aaaagaagga aggtgctcac agccaagcaa ttaaggagta
    11581 agtgtaataa ccgaagtagt ttatgggcta ccgaaataca cttacgccag cattatgaaa
    11641 gtgaatctta cttttgtaaa actttatggt ttgtggaaaa caaatgtttt tgaacattta
    11701 aaaagttcag atgttaaaaa gttgaaaggt taatgtaaa
//
