LOCUS       sequence-49304-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Zhu Y, Xu J, Heinemann SF
  TITLE     Two pathways of synaptic vesicle retrieval revealed by 
            single-vesicle imaging.
  JOURNAL   Neuron. 2009 Feb 12;61(3):397-411.
  PUBMED    19217377
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2009-02-12"; volume: "61(3)"; pages: "397-411"
COMMENT     Sequence Label: pcDNA3-SypHluorin 4x (S4x)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..26)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(42..71)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(86..112)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(588..608)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttaactcaat gatgatgatg atgatggtcg acggcgctat tcagatcctc ttctgagatg
       61 agtttttgtt ctacgtaagc tatcaggcgt aatcgggcac atcgtaaggg taggcagctt
      121 ggtaccgaag cttcggatcc gacatctgat tggagaagga ggtgggcgca ccctgttggc
      181 cgtagccttg ctgcccatag tcgccctgag gcccgtagcc accgccaccg ccgctggctg
      241 gctgcccgta atcgggttga taaccaccct gaggcccgta ggagtcctgg ggcccatagc
      301 ctccggggcc ctgcccatag cccgcatcgc cgtaggcatc gccaggagct ggttgctttt
      361 ctggggcgcc tggaggtgcg cgcatgaatg gggcggccca gcctgtctcc ttgaacacga
      421 accataggtt gccaacccag agcaccaggt tcaggaagcc aaacaccacc gaggtgttga
      481 gtcctgaagt cacagggtcc ctcagttcct tgcatgtgat cgataagctt gatcgagctc
      541 caccggtttt gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa
      601 gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagagtagaa gttgtaaaca
      661 ggtaatggtt gtctggtaaa aggacgggcc catcgccaat tggagtattt tgttgataat
      721 ggtctgctag ttgaacgcct ccatcttcaa tgttgtgtct aattttgaag ttagctttga
      781 ttccattctt ttgtttgtct gccatgatgt acacctggtg atcgttatag ttgtattcca
      841 atttgtgtcc aaga
//
