LOCUS       sequence-493042-       10997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  General screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Datlinger P, Pankevich EV, Arnold CD, Pranckevicius N, Lin J, 
            Romanovskaia D, Schaefer M, Piras F, Orts AC, Nemc A, Biesaga PN, 
            Chan M, Neuwirth T, Artemov AV, Li W, Ladstatter S, Krausgruber T, 
            Bock C
  TITLE     Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
  JOURNAL   Nature. 2025 Oct;646(8086):963-972. doi: 10.1038/s41586-025-09507-9.
            Epub 2025 Sep 24.
  PUBMED    40993398
REFERENCE   2  (bases 1 to 10997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09507-9"; journalName: "Nature"; date: 
            "2025-10"; volume: "646"; issue: "8086"; pages: "963-972"
COMMENT     Sequence Label: pPD0057_CROP-seq-EBFP2
FEATURES             Location/Qualifiers
     source          1..10997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..349
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(530..574)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    759..992
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1485..1610
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1657..1837
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1838..2064)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2092..2112)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2092..2110)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2131..2147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2131..2147)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2144..2166)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2155..2171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2179..2185,2186..2203,2204..2209))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2224..2245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2362..2379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2533..3121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2613..2632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3292..4083,4084..4152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3915..3934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4153..4257)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4283..4738)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4420..4441)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4632..4651
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4865..4887
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4879..4896
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4880..4896
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4906..4925
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4906..4924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4945..5080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5120..5241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5131..5150)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5185..5204
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5319..5499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_RNA        complement(5552..5627)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7509..7757)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7567..7586)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7737..7757)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    7913..8501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8019..8030
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8428..8448
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9237..9293)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9303..9899)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9383..9403)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9880..9899
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(9912..10997,1..93))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9921..10859
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(9944..9964)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tctctaggca ccggatcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga
       61 tgtgcgctct gcccactgac gggcaccgga gccaattccc actcctttca agacctagaa
      121 ggtccattag ctgcaaagat tcctctctgt ttaaaacttt atccatcttt gcacccgggc
      181 cccctcgagc cggcgccaaa gtggatctct gctgtccctg taataaaccc gaaaattttg
      241 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
      301 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa
      361 tactgccatt tgtctcaaga ttctgaaacg ataatggtga atatccctgc ctaactctat
      421 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
      481 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
      541 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
      601 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
      661 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
      721 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
      781 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
      841 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
      901 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
      961 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
     1021 ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat
     1081 atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca
     1141 gcggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
     1201 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     1261 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     1321 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
     1381 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     1441 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
     1501 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
     1561 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
     1621 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
     1681 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     1741 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     1801 gctcccaggc tcagatctgg tctaaccaga gagacccgtt tatgtatcga gctaggcact
     1861 taaatacaat atctctgcaa tgcggcaatt cagtggttcg tccaatccat gtcagacccg
     1921 tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac
     1981 ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact
     2041 acaagagtat tgcataagac tacattaagc ttgcagctcc agcttttgtt ccctttagtg
     2101 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2161 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2221 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2281 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2341 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2461 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2521 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2581 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2641 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2701 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2761 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2821 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2881 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2941 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3001 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3061 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3121 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3181 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3241 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3301 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3361 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3421 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3481 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3541 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3601 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3661 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3721 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3781 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3841 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3901 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3961 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4021 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4081 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4141 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4201 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4261 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     4321 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4381 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4441 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4501 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4561 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4621 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4681 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     4741 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     4801 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     4861 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     4921 tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc tcctcactac ttctggaata
     4981 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     5041 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     5101 tagctagagc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     5161 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     5221 ataagctgca ataaacaagt tcctctcact ctctgatatt catttctttg caagttataa
     5281 atactgaata ataagatgac atgaactact atacgtactg ctagagattt tccacactga
     5341 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     5401 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     5461 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagtgt
     5521 ctcaagatct agttacgcca agcttaaaaa agcaccgact cggtgccact ttttcaagtt
     5581 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacaga gacgtacaaa
     5641 aaagagcaag aagctaaaaa agatttaaaa attattttta gcgcagttaa tggaacagga
     5701 actaaattta ccccaaaaat attacgtgaa tcaggatata acgttattga ggttgaagag
     5761 catgcatttg aagatgaaac atttaaaaat gttgtaaatc caaatccaga atttgatcct
     5821 gcatgaaaaa taccgcttga atatggtatt aaacatgatg cagatattat tattatgaat
     5881 gacccagatg ctgacagatt tggaatggca ataaaacatg atggtcattt tgtaagatta
     5941 gatggaaatc aaacaggacc aattttaatt gattgaaaat tatcaaatct aaaacgctta
     6001 aatagcattc caaaaaatcc ggctctatat tcaagttttg taacaagtga tttgggtgat
     6061 agaatcgctc atgaaaaata tggagttaat attgtaaaaa ctttaactgg atttaaatga
     6121 atgggtagag aaattgctaa agaagaagat aacggattaa attttgtttt tgcttatgaa
     6181 gaaagttatg gatatgtaat tgatgactca gctagagata aagatggaat acaagcttct
     6241 atattaatag cagaggctgc ttgattttat aaaaaacaaa ataaaacatt agtagactat
     6301 ttagaagatt tatttaaaga aatgggtgca tattacactt tcactttaaa cttgaatttt
     6361 aaaccagaag aaaagaaatt aaaaattgaa ccattaatga aatcattgag agcaacaccc
     6421 ttaactcaaa ttgctggact taaagttgtt aatgttgaag actacatcga tggaatgtat
     6481 aatatgccag gacaagactt actaaaattt tatttagaag ataagtcatg atttgctgtt
     6541 cgcccaagtg gaactgaacc taaactaaaa atttatttta taggtgttgg tgaatctgtt
     6601 caaaacgcta aagttaaagt agacgaaatt attaaagaat taaaattaaa aatgaatata
     6661 taggagaaaa aatgaaacta aacaaatata tagatcacac attattaaaa caagatgcta
     6721 cgaaagctga aattaaacaa ttatgtgatg aagcaattga atttgatttt gcaacagttt
     6781 gtgttaattc atattgaaca agctattgta aagaattatt aaaaggcaca aatgtaggaa
     6841 taacaaatgt tgtaggtttt cctctaggtg catgcacaac agctacaaaa gcattcgaag
     6901 tttctgaagc aattaaagat ggtgcaacag aaattgatat ggtattaaat attggtgcat
     6961 taaaagacaa aaattatgaa ttagttttag aagacatgaa agctgtaaaa aaagcagctg
     7021 gatcacatgt tgttaaatgt attatggaaa attgtttatt aacaaaagaa gaaatcatga
     7081 aagcttgtga aatagctgtt gaagctggat tagaatttgt taaaacatca acaggatttt
     7141 caaaatcagg tgcaacattt gaagatgtta aactaatgaa gtcagttgtt aaagacaatg
     7201 ctttagttaa agcagctggt ggagttagaa catttgaaga tgctcaaaaa atgattgaag
     7261 caggagctga ccgcttagga acaagtggtg gagtagctat tattaaaggt gaagaaaaca
     7321 acgcgagtta ctaaaactag cgttttttta ttttgctcat ttttattaaa agtttgcaaa
     7381 aaggaacata aaaattctaa ttattgatac taaagttatt aaaaagaaga ttttggttga
     7441 ttttataaag gtcatagaat ataatatttt agcatgtgta ttttgtgtgc tcatttacaa
     7501 ccgtctccgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt
     7561 caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac
     7621 ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc
     7681 aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc
     7741 atgggaaata ggccctctct gggtcccctc ggggttggga ggtgggtctg aaacgataat
     7801 ggtgaaatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa
     7861 aaagtggcta agatctacag ctgccttgta agtcattggt cttaaagtcg acgcggggag
     7921 gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc
     7981 agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag
     8041 cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca aggaaaggac
     8101 gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag
     8161 cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa
     8221 gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca
     8281 aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac
     8341 agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga
     8401 tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata
     8461 ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgtcgactta acgcgttcac
     8521 ttgtacagct cgtccatgcc cagggtgatg ccggcggcgg tcctgaactc cagcagcacc
     8581 atgtggtccc tcttctcgtt ggggtccttg ctcagcacgc tctgggtgct caggtagtgg
     8641 ctgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcc
     8701 agctgcacgc tgccgtcctc cacgttgtgc ctgatcttga agttcacctt gatgccgttc
     8761 ttctgcttca cggccatgat gtagatgttg tggctgttga agttgtactc cagcttgtgg
     8821 cccaggatgt tgccgtcctc cttgaagtcc acgcccttca gctcgatcct gttcaccagg
     8881 gtgtcgccct cgaacttcac ctcggccctg gtcttgtagg tgccgtcgtc cttgaagaag
     8941 atggtcctct cctgcacgta gccctcgggc atggcgctct tgaagaagtc gtgctgcttc
     9001 atgtggtcgg ggtacctggc gaagcactgc acgccgtggc tcagggtggt caccagggtg
     9061 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgttg
     9121 gtggcgtcgc cctcgccctc gcccctcacg ctgaacttgt ggccgttcac gtcgccgtcc
     9181 agctccacca ggatgggcac cacgccggtg aacagctcct cgcccttgct caccatagga
     9241 ccggggtttt cttccacgtc gcctgcttgc tttaacagag agaagttcgt ggctccggat
     9301 ccggcaccgg gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg
     9361 gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc
     9421 aggaaggcgg gcaccccggc gcgctcggcc gcctccactc cggggagcac gacggcgctg
     9481 cccagaccct tgccctggtg gtcgggcgag actccgacgg tggccaggaa ccacgcgggc
     9541 tccttgggcc ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc cagccgggaa
     9601 ccgctcaact cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc
     9661 tccggcgtgg tccagaccgc cacggcggcg ccgtcgtccg cgacccacac cttgccgatg
     9721 tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc gatgtggcgg
     9781 tccggatcga cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt
     9841 acggccctgg ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg
     9901 gtggccgtac gtcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc
     9961 ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag
    10021 tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa
    10081 aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc
    10141 ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc
    10201 cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt
    10261 gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac
    10321 cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga
    10381 gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc
    10441 cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa
    10501 accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa
    10561 agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga
    10621 caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca
    10681 actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt
    10741 ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggtggaagt aattcaaggc
    10801 acgcaagggc cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc
    10861 tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata
    10921 tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag
    10981 tttaccccgc gccacct
//
