LOCUS       sequence-49305-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Zhu Y, Xu J, Heinemann SF
  TITLE     Two pathways of synaptic vesicle retrieval revealed by 
            single-vesicle imaging.
  JOURNAL   Neuron. 2009 Feb 12;61(3):397-411.
  PUBMED    19217377
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2009-02-12"; volume: "61(3)"; pages: "397-411"
COMMENT     Sequence Label: pcDNA3-SypHluorin 4x (S4x)
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     638..658
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     regulatory      738..747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
       61 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      121 ttatttgcac tactggaaaa ctacctgttc cttggccaac acttgtcact actttaactt
      181 atggtgttca atgcttttca agatacccag atcatatgaa acggcatgac tttttcaaga
      241 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      301 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      361 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      421 acgatcacca ggtgtacatc atggcagaca aacaaaagaa tggaatcaaa gctaacttca
      481 aaattagaca caacattgaa gatggaggcg ttcaactagc agaccattat caacaaaata
      541 ctccaattgg cgatgggccc gtccttttac cagacaacca ttacctgttt acaacttcta
      601 ctctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      661 ctgctgggat tacacatggc atggatgaac tatacaaaac cggtggagct cgaatctgca
      721 gaattcgccc ttggctagcc accatgggan gtaaaggaga agaacttttc actggagttg
      781 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      841 agggtgaagg tgatgcaact tacgg
//
