LOCUS       sequence-493071-       10310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet- or Dox-inducible expression of PABPC1 fused to INT (human PARP4
            fragment).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10310)
  TITLE     A Genetically Encoded Device for Transcriptome Storage in Mammalian 
            Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKC105_pLenti_TetON_PABPC1_INT
FEATURES             Location/Qualifiers
     source          1..10310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    80..197
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(490..534)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    719..952
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1449..1574
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1621..2254
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2446..2465)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2515..2531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2515..2531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2528..2550)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2539..2555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2563..2569,2570..2587,2588..2593))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2608..2629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2869..2886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3040..3628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3120..3139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3799..4590,4591..4659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4422..4441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4660..4764)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4832..4850
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4969..5202
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5277..5865
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5383..5394
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5792..5812
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5878..6624)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6625..6678)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6685..7395)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      7392..7401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             7436..7704
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(7717..7928)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     8114..8133
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    8132..8187
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     8233..8252
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     regulatory      9882..9891
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(10016..10040)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10085..10103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10121..10139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10157..10175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10193..10211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10229..10247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10265..10283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    join(10301..10310,1..9)
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgatagggag taatctagtg gccgtggagg ccaccggtct agtccaaact ggatctctgc
       61 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
      121 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
      181 ccccttttct tttaaaattg tggatgaata ctgccatttg tgaattcggc gataccgtcg
      241 agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat
      301 tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
      361 atatccctgc ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac
      421 caagcctcct actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa
      481 ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg
      541 ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct
      601 gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact
      661 agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag
      721 gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat
      781 gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt
      841 ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc
      901 tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac
      961 tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg
     1021 ttcaattttt actactttat atttatataa ttcacttctc caattgtccc tcatatctcc
     1081 tcctccaggt ctgaagatca gcggccggcc gcttgctgtg cggtggtctt acttttgttt
     1141 tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc
     1201 acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga
     1261 agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca
     1321 ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat
     1381 tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc
     1441 ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt
     1501 ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc
     1561 ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac
     1621 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     1681 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     1741 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     1801 cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca ctccccagtc
     1861 ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta gcaagctcga
     1921 tatcagcagt tcttgaagta ctccggatgc agctctcggg ccacgtgatg aaatgctagg
     1981 cggctgtcaa acctccactc taacacttct ctctccgggt catccatccc atgcaggctc
     2041 acagggtgta acaagctggt gttctctcct ttattggcct cttctacctt atctggctca
     2101 actggtacta gcttgtagca ccatccaaag gtcagtggat atctgacccc tggccctggt
     2161 gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt agatccacag atcaaggata
     2221 tcttgtcttc tttgggagtg aattagccct tccaactact aagtttgtag tacatattta
     2281 acaaatacaa tttctttaaa atgaaaataa ttcagaggaa tcacaggttt agagtaaatg
     2341 aaaccacagg taattggcag tggtaatagg gtatggggtg ggaagtttgg gatgattttg
     2401 gttagcttga gttatccagt tgatccagac atgataagat acattgatga gtttggacaa
     2461 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaattcgtaa tcatgtcata
     2521 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2581 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2641 ctcactgccc gctttccagt cgggaaacct gtcgtgccac ggggagcttt ttgcaaaagc
     2701 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     2761 cggcctctgc ataaataaaa aaaattagtc agccatgagc ttgctgcatt aatgaatcgg
     2821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4381 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     4441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     4801 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4861 tcaagaatga tctagccctt tccttaatta acccgggcct ctcactctct gatattcatt
     4921 tctttgcaag ttataaatac tgaataataa gatgacatga actactactg ctagagattt
     4981 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     5041 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     5101 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa
     5161 aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta
     5221 aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaagga tctcaggcgg
     5281 ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg
     5341 ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac
     5401 gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa
     5461 ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt
     5521 ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc
     5581 gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc
     5641 agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag
     5701 agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc
     5761 atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag
     5821 aataccagtc aatctttcac aaattttgta atccagaggt tgattaggat cgtcgactta
     5881 cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc
     5941 aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggt
     6001 cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc
     6061 gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt gtttcagaag
     6121 tgggggcata gaatcggtgg taggtgtctc tctttcctct tttgctactt gatgctcctg
     6181 ttcctccaat acgcagccca gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt
     6241 ctccagggag aagccttgct gacacaggaa cgcgagctga ttttccaggg tttcgtactg
     6301 tttctctgtt gggcgggtgc cgagatgcac tttagccccg tcgcgatgtg agaggagagc
     6361 acagcggtat gacttggcgt tgttccgcag aaagtcttgc catgactcgc cttccagggg
     6421 gcaggagtgg gtatgatgcc tgtccagcat ctcgattggc agggcatcga gcagggcccg
     6481 cttgttcttc acgtgccagt acagggtagg ctgctcaact cccagctttt gagcgagttt
     6541 ccttgtcgtc aggccttcga taccgactcc attgagtaat tccagagcag agtttatgac
     6601 tttgctcttg tccagtctag acattgggcc aggattctcc tcgacgtcac cgcatgttag
     6661 cagacttcct ctgccctcgc tagcattaag tttgtgcccc agtttgctag ggaggtcgca
     6721 gtatctggcc acagccacct cgtgctgctc gacgtaggtc tctttgtcgg cctccttgat
     6781 tctttccagt ctgtggtcca catagtagac gccgggcatc ttgaggttct tagcgggttt
     6841 cttggatctg tatgtggtct tgaagttgca gatcaggtgg cccccgccca cgagcttcag
     6901 ggccatgtcg cttctgcctt ccaggccgcc gtcagcgggg tacagcatct cggtgttggc
     6961 ctcccagccg agtgttttct tctgcatcac agggccgttg gatgggaagt tcacccctct
     7021 gatcttgacg ttgtagatga ggcagccgtc ctggaggctg gtgtcctggg tagcggtcag
     7081 cacgcccccg tcttcgtatg tggtgactct ctcccatgtg aagccctcag ggaaggactg
     7141 cttaaagaag tcggggatgc cctgggtgtg gttgatgaag gttctgctgc cgtacatgaa
     7201 gctggtagcc aggatgtcga aggcgaaggg gagagggccg ccctcgacca ccttgattct
     7261 catggtctgg gtgccctcgt agggcttgcc ttcgccctcg gatgtgcact tgaagtggtg
     7321 gttgttcacg gtgccctcca tgtacagctt catgtgcatg ttctccttaa tcagctcttc
     7381 gcccttagac accatggtgg cgttggctgc aggtgtaggc gccggtcaca gcttggatct
     7441 gtaacggcgc agaacagaaa acgaaacaaa gacgtagagt tgagcaagca gggtcaggca
     7501 aagcgtggag agccggctga gtctaggtag gctccaaggg agcgccggac aaaggcccgg
     7561 tctcgacctg agctttaaac ttacctagac ggcggacgca gttcaggagg caccacaggc
     7621 gggaggcggc agaacgcgac tcaaccggcg tggatggcgg cctcaggtag ggcggcgggc
     7681 gcgtgaagga gagatgcgag cccctcgaag cttcagctgt gttctggcgg caaacccgtt
     7741 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     7801 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     7861 ggcacccgtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     7921 ctctgcccac tgacgggcac cggagcgatc gcagatcctt actagtcata tgtaaggaat
     7981 ggacagcagg gggctgtttc atatactgat gacctcttta tagccaacct ttgttcatgg
     8041 cagccagcat atgggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta
     8101 aatgatttgc cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta
     8161 ttgcaatgaa aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga
     8221 tgtccccata atttttggca gagggaaaaa gattaggcgg ccgctcagtt atctagatcc
     8281 ggtggatcct tagccctggg agtaatgcag gactcgatgc agtggtgaca ctgttgaaat
     8341 tggttgcaga cccaagagct gcttggttgc gctatcccag tcgttcccca actccagtcg
     8401 gggacatatg cttgggtact gaccctctgt tcgcctgacc cactcactcg cctgcttgat
     8461 ggcctcaaaa gcccagggga tgtttctact tatactagcg tcgtccattt tcatcagact
     8521 tttgaatacg ataccctctt tttccagcct agtgcggata aattgcaaca cgagcatcgt
     8581 ggctatgagg tcgagaaggc actcccggcc cttaacaccg agactctgga tgcctttctg
     8641 cttcagaaag gagtggagcc catttgtatt aaggttcaat atgagtccga gttcgggcgt
     8701 gagcttccag aaaccatcct ccgtctggag agacagcaat tccgtccaag gaacggcatc
     8761 ttgccagtgc tggatgcaca cgctaccacc gcgctcttct ttgcgctgag ctaaagctac
     8821 atacaatggc tttgtggcca caattctacc gttcatttct gtaactgctt tagtggcttc
     8881 ttctggggag gagaaacata caaaaccaaa ccctttgctg cgaccaccct ccatcataac
     8941 ctttgcacta gtgattgtac caaatggaga aaactctttc cggagacgtt catcatcaat
     9001 accatcatca agatttttca cataaagatt aacaccctgg tatctggtga tcctatcttg
     9061 tttcatctgt tcaaatttgc gcttaagttc cgtctgccgt tccacctttt tctgagctcg
     9121 accaacataa atttgttttc cattgagctc ctttccgttc atctcatcca cagctttctg
     9181 tgcatcttca tgcctttcaa agcttacaaa tccaaatcct ttggattttc cactttcatc
     9241 agtcattact ttcacactta aggcaggccc aaacttgcca aagagatcct taaggcgctc
     9301 atcatccatg tcttctccaa aattcttgat gtaaacattg gtgaattctt ttgccctagc
     9361 tccaagttca gcttctcgtt ctttacgaga cttaaatcgt ccaacaaata ctttgcgatc
     9421 atttaggagc attccattca ttttttcaat agctctttca gctgcttcct gcgtctcaaa
     9481 gtgtacaaat ccatagccct tggaaccatt ttcatcacaa accaccttac atgaaaggat
     9541 gttaccaaaa gcagaaaatg tatcatacag tgctttatta tcaatggatt tgtccagatt
     9601 tttaatgaat atgttgccta ctccactttt gcgaagtgat ggatcacgct gagaccacat
     9661 gatgcgtact ggcttgccct ttataacatc aaaattcatg gtgtccaaag cacgctccgc
     9721 gtccgccggc tgctggaagt tcacatacgc gtagcccaag gagcggcggg tgatcatgtc
     9781 cctgcagacc cggatggaga ggatgggccc ggccgggctg aacttctcgt agagcatcgc
     9841 ctcggtcacg tcggggtgga ggtcccccac gtagagcgag gccatggtgg cggctcgagc
     9901 gaagttccta taccttttga agaataggaa cttcggaata ggaacttctt tacgagggta
     9961 ggaagtggta cggaaagttg gtataagaca aaagtgttgt ggaattgctc caggcgatct
    10021 gacggttcac taaacgagct ctgcttttat aggcgcccac cgtacacgcc taaagcttat
    10081 acgttctcta tcactgatag ggagtaaact ggatatacgt tctctatcac tgatagggag
    10141 taaactgtag atacgttctc tatcactgat agggagtaaa ctggtcatac gttctctatc
    10201 actgataggg agtaaactcc ttatacgttc tctatcactg atagggagta aagtctgcat
    10261 acgttctcta tcactgatag ggagtaaact cttcatacgt tctctatcac
//
