LOCUS       sequence-493094-        8177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that can express uTEV3. Expressed off of CMV tet 
            ON promoter with EBFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K,
            Tiwari R, Mukherjee A
  TITLE     A programmable genetic platform for engineering noninvasive 
            biosensors
  JOURNAL   bioRxiv 2025.09.07.674790
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.07.674790"
COMMENT     Sequence Label: pNTA-uTEV3-GSG-t2A-EBFP
FEATURES             Location/Qualifiers
     source          1..8177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    81..206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             253..433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(434..660)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(688..708)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(688..706)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(727..743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(727..743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(740..762)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    751..767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(775..781,782..799,800..805))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    820..841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(958..975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1129..1717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1209..1228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1888..2679,2680..2748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2511..2530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2749..2853)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2879..3334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3016..3037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3228..3247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3461..3483
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3475..3492
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3476..3492
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3502..3521
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3502..3520
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3541..3676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3716..3837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3727..3746)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3781..3800
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3909..4142
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4275..4863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4381..4392
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4790..4810
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    4894..4918
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4943..5656,5657..5659))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKV
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLLE
                     FRTAAGITLGMDELYK"
     primer_bind     5596..5617
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5660..5713)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5723..6430)
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGTFWKHWIQTKDGQCGNPLVSTRDGFI
                     VGIHSASNFTNTNNYFASVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     misc_feature    6471..6523
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(6552..6576)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6657..6681)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6678..6716)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    6728..6746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6764..6782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6799..6817
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6835..6853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6871..6889
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    6919..7036
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7303..7347)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7532..7765
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
       61 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
      121 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
      181 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
      241 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
      301 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
      361 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
      421 accagagaga cccgtttatg tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg
      481 gcaattcagt ggttcgtcca atccatgtca gacccgtctg ttgccttcct aataaggcac
      541 gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct ccttgtaagg
      601 catgttgcta actcatcgtt accatgttgc aagactacaa gagtattgca taagactaca
      661 ttaagcttgc agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
      721 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      781 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      841 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      901 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      961 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1021 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1081 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1141 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1201 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1261 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1321 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1381 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1441 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1501 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1561 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1621 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1681 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1741 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1801 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1861 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1921 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1981 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2041 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2101 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2161 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc
     2221 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2281 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2341 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2401 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2461 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2521 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2581 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2641 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2701 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2761 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2821 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     2881 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     2941 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     3001 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     3061 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     3121 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3181 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3241 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3301 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     3361 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     3421 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     3481 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacgtcccta
     3541 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     3601 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggct
     3661 gagttagggg cgggatgggc ggagttaggg gcgggatagc tagagccaga catgataaga
     3721 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3781 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttcct
     3841 ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga
     3901 actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     3961 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     4021 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     4081 gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgag ctagcccttc
     4141 cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg
     4201 tcttaaaggt accgggccct cgacggatcc ccggtagaat tagatcacta gtccggatat
     4261 ctgcagaatt ccaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     4321 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     4381 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     4441 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
     4501 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     4561 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     4621 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     4681 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     4741 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     4801 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     4861 attgtcgagc ggccgccact gtgctggata tcaaccactt tgtacaagaa agctgggtct
     4921 agatatctcg agtgcggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg
     4981 cggcggtgcg gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     5041 gcacggactg ggtgctcagg tagtggctgt cgggcagcag cacggggccg tcgccgatgg
     5101 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctccacg ttgtggcgga
     5161 tcttgaagtt caccttgatg ccgttcttct gcttgacggc catgatatag atgttgtggc
     5221 tgttgaagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgacgc
     5281 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     5341 tgtaggtgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     5401 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cactgcacgc
     5461 cgtggctcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     5521 tgaacttcag ggtcagcttg ccgttggtgg catcgccctc gccctcgccc ctcacgctga
     5581 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     5641 gctcctcgcc cttgctcact gggccaggat tctcttccac gtctccgcag gtgagcaggc
     5701 tgcctctacc ctcgccggat ccattcatga gttgagtcgc ttccttaact ggctgaaaag
     5761 gctcttcagg tttcaccatg aaaactttat ggccccccca caatactgag tcagcattta
     5821 atcgccaacc actaacccac tgctgcgcct cctgatttgt caacaattcc atgaagtttt
     5881 tcggcacgct tgcgaaataa ttgtttgtgt tggtgaaatt cgatgctgag tgtataccaa
     5941 caatgaaccc atctctagtt gatactaatg gattgccaca ctgcccatcc ttggtttgaa
     6001 tccaatgctt ccagaatgtg ccatcagatg aagggaatgt gcaactagtg tctgacacca
     6061 tgctagacat gctcttagtt tggaagttgg ttgtcacaag acatatgcgc tcttcccttt
     6121 gtggctctct aaatttcagc ttttgaggaa atggtgggaa atccttaggc atgcgaataa
     6181 ttatcatgtc cctcccatca atgaggtgtt gttgcaaagt cgtggtgttc ttgaccttga
     6241 atacaccatg tagtgattgg accaacagtg ttccattatt tcttctaaac aagtgcttgt
     6301 ttgtaatgat gaagggacca aatccaatac catacaacga tgttgtgtgc ccatcagatt
     6361 cattcgtcaa atgacaaatg gtgctcgata tcgggttgta atcacgcggc cccttaaaca
     6421 agctttctcc catggtgaag ggtactggat ccggctcggt ggcgaccggt ggatcccggg
     6481 cccgcggtac cgtcgactgc agaattcgaa gcttgagctc gagatccagt cgactgaatt
     6541 ggttccttta aagcctgctt ttttgtacaa acttgttgat atctgcagaa ttccaccaca
     6601 ctggactagt cgacgaaccg gtggatcccc gggtaccgag ctcgaattct ccaggcgatc
     6661 tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg cctacctcga
     6721 catacgttct ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg
     6781 gataaactcg acatacgttc tctatcactg atagggagta aactcgacat acgttctcta
     6841 tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgag
     6901 gctagtctcg tgatcgataa aattttgaat ttttgtaatt tgtttttgta attctttagt
     6961 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     7021 cccttttctt ttaaaagtta accgataccg tcgagatccg ttcactaatc gaatggatct
     7081 gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg
     7141 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
     7201 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
     7261 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
     7321 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga
     7381 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
     7441 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca
     7501 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc
     7561 cacagccagg attctcgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg
     7621 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa
     7681 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc
     7741 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct
     7801 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata
     7861 taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg
     7921 cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct
     7981 tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata
     8041 atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc
     8101 tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc
     8161 ttgcccatac tatatgt
//
