LOCUS       sequence-49318-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, 
            Tsien RY
  TITLE     Ca2+ indicators based on computationally redesigned 
            calmodulin-peptide pairs.
  JOURNAL   Chem Biol. 2006 May;13(5):521-30.
  PUBMED    16720273
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2006-05"; volume: "13(5)"; pages: "521-30"
COMMENT     Sequence Label: pcDNA-lynD3cpV
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(61..573,574..576,577..579))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     254..273
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     513..534
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(637..658)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc agaattctta
       61 ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt
      121 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      181 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      241 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      301 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      361 ggcgaagcac tgcaggccgt agcccagggt ggtcacgagg gtgggccagg gcacgggcag
      421 cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat cgccctcgcc
      481 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      541 caccaccccg gtgaacagct cctcgccctt gctcaccatg ccaccgctgc cacccttgta
      601 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      661 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc
      721 gggcagcagc acggggccgt cgccgatggg ggtgttct
//
