LOCUS       sequence-49319-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: PIGAZFN-L2
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             126..149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcacca tgggacctaa gaaaaagagg aaggtggcgg
      121 ccgctgacta caaggatgac gacgataaat ctagacccgg ggagcgcccc ttccagtgtc
      181 gcatttgcat gcggaacttt tcggacggca gcacgcttaa caggcatacc cgtactcata
      241 ccggtgaaaa accgtttcag tgtcggatct gtatgcgaaa tttctcccag gggagcagct
      301 tgaggcggca tctacgtacg cacaccggcg agaagccatt ccaatgccga atatgcatgc
      361 gcaacttcag tatcagccac aacctggcgc ggcacctaaa aacccacctg aggggatccc
      421 aactagtcaa aagtgaactg gaggagaaga aatctgaact tcgtcataaa ttgaaatatg
      481 tgcctcatga atatattgaa ttaattgaaa ttgccagaaa ttccactcag gatagaattc
      541 ttgaaatgaa ggtaatggaa ttttttatga aagtttatgg atatagaggt aaacatttgg
      601 gtggatcaag gaaaccggac ggagcaattt atactgtcgg atctcctatt gattacggtg
      661 tgatcgtgga tactaaagct tatagcggag gttataatct gccaattggc caagcagatg
      721 aaatgcaacg atatgtcgaa gaaaatcaaa cacgaaacaa acatatcaac cctaatgaat
      781 ggtggaaagt ctatccatct tctgtaacgg aatttaagtt tttatttgtg agtggtcact
      841 ttaaaggaaa ctacaaagct cagcttacac gattaaatca tatcactaat tgtaa
//
