LOCUS       sequence-49321-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Palmer AE, Jin C, Reed JC, Tsien RY
  TITLE     Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed 
            with an improved genetically encoded fluorescent sensor.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Epub 2004 Dec
            7.
  PUBMED    15585581
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-12-14"; volume: "101(50)"; pages: 
            "17404-9. Epub 2004 Dec 7"
COMMENT     Sequence Label: pcDNA-D1cpV
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..804)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     263..282
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     522..543
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(646..667)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcttac tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt
      121 gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      181 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      241 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      301 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      361 ggggtagcgg gcgaagcact gcaggccgta gcccagggtg gtcacgaggg tgggccaggg
      421 cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt aggtggcatc
      481 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      541 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgc caccgctgcc
      601 acccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      661 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt agctcaggta
      721 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      781 ggcgagctgc acgccgccgt ccatgagctc cagt
//
