LOCUS       sequence-493218-       12295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optimized tetrathionate sensor with recombinase switch and 
            fluorescent reporter (GFP), constitutive mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 42 to 76)
  AUTHORS   Buss MT, Zhu L, Kwon JH, Tabor JJ, Shapiro MG
  TITLE     Probiotic acoustic biosensors for noninvasive imaging of gut 
            inflammation.
  JOURNAL   Nat Commun. 2025 Aug 25;16(1):7931. doi: 10.1038/s41467-025-62569-1.
  PUBMED    40855083
REFERENCE   2  (bases 1 to 12295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-08-25"; volume: "16(1):7931. doi"; pages: " 
            10.1038/s41467-025-62569-1"
COMMENT     Sequence Label: MB 103C ttrSR(m13)-Bxb1_P7-sfGFP_mCherry
FEATURES             Location/Qualifiers
     source          1..12295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..76
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             86..97
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             105..815
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(250..268)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     518..537
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2093..2127
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
     regulatory      3255..3264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      4196..4267
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4283..4310
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     4377..4395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4416..4450
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     terminator      5121..5192
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5208..5235
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             5531..7030
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSSVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      complement(7408..7437)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     protein_bind    7489..7538
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    7593..7640
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     misc_RNA        7647..7696
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             7754..7765
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7772..8485
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(8508..8525)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8678..8764
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8856..8883
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        8902..8993
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9451..9906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9538..9557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9748..9769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(10462..11121)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWCEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     11036..11055
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(11122..11224)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(11616..11633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11750..12295)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(11830..11849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcatc ttattaatca gataaaatcg ccagatatcg attgacggct agctcagtcc
       61 taggtacagt gctagcgaat tcattaaaga ggagaaaggt acccatggtg agcaagggcg
      121 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      181 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      241 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
      301 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      361 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      421 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      481 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      541 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
      601 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
      661 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
      721 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
      781 actccaccgg cggcatggac gagctgtaca agtaagcttt ctaactaaaa aggcctccca
      841 aatcgggggg ccttttttat tgataacaaa acgctcgtct agggttattt cgatgaaagg
      901 agagagtact aatgccaatt gcaataggaa ataaacggtt acccgttacg ttggatgaaa
      961 agagacaaaa agaattgcag caactaaagc agaagtacgg caaaagtgaa tccaggatta
     1021 tgtgtattgc gttagattta ttgattgccc aagaaaaagc aggatttgag gtaccagcac
     1081 tcaaaaagtg acgtcacttt ttatcctaaa aactaaaagt gatagcactt ttaattataa
     1141 gaagttagaa tattaatcat ttgcttaatt gtacaatata atgtacaatt gttttataga
     1201 aataaataag gggtgaaagg aatggaagca gtagcttatt caaatttccg ccaaaattta
     1261 cgtagttata tgaaacaagt taatgaggat gctgaaacac ttattgtaac aagtaaagat
     1321 gtagaagata cagttgttgt attatcaaaa agagattatg attctatgca agaaacgttg
     1381 agaacacttt ctaataatta cgtcatggaa aaaattcgtc gaggagatga acaattctcc
     1441 aaaggtgcat ttaaaacaca tgacttaatc gaggttgaat ctgatgatta aggcttggtc
     1501 tgatgatgct tgggatgatt atctttattg gcatgagcaa ggaaacaaaa gcaatataaa
     1561 aaagattaac aagttaataa aagatatcga tcgttccccc tttgctggat taggaaaacc
     1621 tgagccatta aagcatgatt tatctggaaa atggtccaga agaattacag atgaacatag
     1681 actgatatat agagttgaaa atgaaacgat atttatttat tctgcaaaag atcactatta
     1741 accaatcgga agtaaggaaa gggtcagaaa cttaaaagtt tttgatcctt attttattta
     1801 ccctagtcat ttaaaaagct aatatagctt agtgttgatt gttattaatg aatgtgtttg
     1861 ttacgcgtat tacggatata aggttagtaa aatcatttct aaagttgagg aaaagtaaat
     1921 ataaatggct taaatttcaa caatttgaag ttgaatagat atgttataat actattgtag
     1981 tgtgggatgt tagttactaa aggatgacgc ttatatatat gactgaatag aataagcaat
     2041 aggtttaata atctatttta aattttttgt actagtttta gttaattagc ggtttacagc
     2101 tagctcagtc ctagggactg tgctagcata atcattaaca acatcttata tcaaatttta
     2161 atatgttttc ttccggcatg agcctgaaat ggcctctgag catccagggt tccatcgaac
     2221 cgaaaatgac tccgcgcatc atcttccgca tggccctgcg cactaaagtg ttcatcaccc
     2281 tgctggcttg cctgtttgct tctgccaacg tattcgcggt ggaaccgcag aaccagtccg
     2341 cgccttccga tgtgatggac gctgaggtgg ttgctccggt tgcgacggag gaaccgggtt
     2401 accaggtcgt tgatgttggt gtgctggcga ttcgcggtta taaagctacc atcaaccgtt
     2461 ggcagccact gatgggttgg ctggagactc agatcccgaa ctcctacttc cgcctgcacc
     2521 cgctgtctct ggacgagctg gcaaagggtg tagaaaccca gggtctggac ttcgtaatca
     2581 ccaatccagg ccagtccgta ctgctggcgc gtcagtattc tctgacgtgg ctggcgaccc
     2641 tgcgctctcc gctgaacaac ggtgctgcga tgcaggtggg ttccgcgctg gtagttcgtg
     2701 ccgacagccc gtaccagacc ctgaccgatc tgaaaggccg tcgtctgggc atcgtcagca
     2761 aaaacgcatt cggtggttat ctgacgctgg tatatgaggc acagctgaaa ggcatcgatc
     2821 tgccgcgctt tgttggtgaa atcatcccac tgggcttccc gctggataac ctgctgtatc
     2881 agctggatga tcagaaatct ggtaatgacg ccgcaaaaga caatcgtctg gatgccaccg
     2941 ttgtaccggt atgccaactg gaacagatgc aggccgaagg tctgatcaac atcggccact
     3001 atcgcgttct ggacaaccag accccggtag gtttccactg tcaagtaagc actcgtctgt
     3061 atccgaactg gtctatggct aaaactaacc gtgccagcca gtccctggca aaatctgtga
     3121 cccaggctct gctggctctg ccggaagatc acctggcagc gaaaagctct gactctgcag
     3181 gttggaccac tgcagtgtcc cagctggcga tcgaccagct gctgaaagac ctggacatgc
     3241 atccactgca gaccccatgg tggcagcgcg cttggcagtg ggtaaaactg caccagcagt
     3301 gggcgtggtt cattctgggt atcctggtcc tgctgaacgc ataccatttc tggctggaat
     3361 accgtttctc ccgtcgtggt cgtgaactga tcaacaccca gcgccagctg aacgaaaacc
     3421 gtgctctgct ggagcacgca caacgtatcg ccatcgctgg cgaactgggt gctagcctgt
     3481 cccatgaact gaaccagccg ctggctgcaa ttggtcacta ttgccatggt gcggaagtgc
     3541 gtctgcagcg tggtacttct cctgaagaac tgcagtctgt tctgaccctg atccagcagg
     3601 aagttacgcg cgccgactct atcatcagcc gtctgcgtaa cctgctgaaa aaacgcccgg
     3661 ttagcaaaca ggctctgtat ctgcacgaac tggtaaacga aacggtgccg ctgctggcgt
     3721 acgaattcga acagcaccag atcaacctgg cggtaaacgt cagcggtgaa ccttacctgc
     3781 aatctctgga cgaggtcggt atgcagcagc tgctgctgaa cctgctgaaa aacgcgtttg
     3841 acgcgtgcgt tcagcgcctg gaactggaat cctccggcac tgaacagagc ggtatgcgta
     3901 aaccgtatgt tcctacgatc gatattgatc tgcgctacca ggagcgtact ctgctgctga
     3961 ctgtgacgga taacggcact ggtctgaccg aggagactag cctgctgatg caggccttct
     4021 attccaccaa atctgaaggc ctgggcctgg gtctggttat ttgccgtgat atcgctgagt
     4081 cccacggtgg caccttctct ctggaatccg ccatgggtgg tggctgccag gctcaggtgg
     4141 ccatcccgcg caagccggaa ccgaatggcg ttctgtaata atctagacca ggcatcaaat
     4201 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     4261 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggta
     4321 cccggggatc ctctagcgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4381 ggcgtatcac gaggcccttt cgtcttcacc tcgagttgat agctagctca gtcctaggta
     4441 ttgtgctagc accaaatata gagaatcaca aggatctatt caatgagcct ggcgctgccg
     4501 gtgtacctga ttgacgacga tgactccgtt cgccgttccc tgcgtttcat gctggaaagc
     4561 tacggcctga aaatcactga cttcgattct gctgaagcgt tcttcaccgc ggtagacctg
     4621 accctgccgg gttgcgcact ggtggacgta cgtatgccgg gcctgagcgg cccacagctg
     4681 cacctggaac tggtgtctaa gaacagcccg ctggccgtga tctatctgac cggtcacggc
     4741 gacgttccga tggcggttga agcgctgaaa ctgggtgcgg tagatttctt tcagaaacct
     4801 gcagacggcg ctaaactggc tgaagctgtg gtcaaagccc tggaacacgc gaaaacccac
     4861 taccaagaca accagtacct ggaaacctat caggctctga ccccacgtga gcgcgaaatc
     4921 ctgaacctga ttgcgcaagg tctgaaaaac caggaaatcg cggacaacct gtgcattgcg
     4981 atgcgcaccg tagaaatcca ccgtgcgaac ctgatgaaag gtatgcaggt gggcagcctg
     5041 gcagagatga tgctgattta ctctcgtatc gcggagcgtc tgcgcctgga agataaccgt
     5101 taataatcta gaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     5161 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     5221 gtgggccttt ctgcgtttat agtaaatcac tgcataattc gtgtcgctca aggcgcactc
     5281 ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat
     5341 gagctggtta gctagtcaat atttgttgcg ctagatcaaa tccacgcctg atatgtggaa
     5401 aaccactata gttatgccgc ctcgcctttt acagaatgcc tgtacgcact gaccaccatg
     5461 aaggtgacgc tcatgactta cttgctgaat ctcaggagtt tatgaccttt tttttttaga
     5521 tcctatcggt atgagagccc tggtagtcat ccgcctgtcc agcgtcaccg atgctacgac
     5581 ttcaccggag cgtcagctgg agtcttgcca gcagctctgc gcccagcgcg gctgggacgt
     5641 cgtcggggta gcggaggatc tggacgtctc cggggcggtc gatccgttcg accggaagcg
     5701 cagaccgaac ctggcccggt ggctagcgtt cgaggagcaa ccgtttgacg tgatcgtggc
     5761 gtaccgggta gatcggttga cccgatcgat ccggcatctt cagcagctgg tccactgggc
     5821 cgaggaccac aagaagctgg tcgtctccgc gaccgaagcg cacttcgata cgacgacgcc
     5881 gtttgcggcg gtcgtcatcg cgcttatggg aacggtggcg cagatggaat tagaagcgat
     5941 caaagagcgg aaccgttcgg ctgcgcattt caatatccgc gccgggaaat accgaggatc
     6001 cctgccgccg tggggatacc tgcctacgcg cgtggacggg gagtggcggc tggtgccgga
     6061 ccctgtgcag cgagagcgca tcctcgaggt gtatcaccgc gtcgtcgaca accacgagcc
     6121 gctgcatctg gtggcccacg acctgaaccg gcgtggtgtc ctgtcgccga aggactactt
     6181 cgcgcagctg caaggccgcg agccgcaggg ccgggagtgg tcggctaccg cgctgaagcg
     6241 atcgatgatc tccgaggcga tgctcgggta cgcgactctg aacggtaaga ccgtccgaga
     6301 cgacgacgga gccccgctgg tgcgggctga gccgatcctg acccgtgagc agctggaggc
     6361 gctgcgcgcc gagctcgtga agacctcccg ggcgaagccc gcggtgtcta ccccgtcgct
     6421 gctgctgcgg gtgttgttct gcgcggtgtg cggggagccc gcgtacaagt tcgccggggg
     6481 aggacgtaag cacccgcgct accgctgccg ctcgatgggg ttcccgaagc actgcgggaa
     6541 cggcacggtg gcgatggccg agtgggacgc gttctgcgag gagcaggtac tggatctgct
     6601 cggggacgcg gagcgtctgg agaaagtctg ggtagcgggc tcggactccg cggtcgaact
     6661 cgcggaggtg aacgcggagc tggtggacct gacgtcgctg atcggctccc cggcctaccg
     6721 ggcgggctct ccgcagcgag aagcactgga tgcccgtatt gcggcgctgg ccgcgcggca
     6781 agaggagctg gagggcctgg aggctcgccc gtctggctgg gagtggcgcg agaccgggca
     6841 gcggttcggg gactggtggc gggagcagga caccgcggca aagaacacct ggcttcggtc
     6901 gatgaacgtt cggctgacgt tcgacgtccg cggcgggctg actcgcacga tcgacttcgg
     6961 ggatcttcag gagtacgagc agcatctcag gctcggcagc gtggtcgaac ggctacacac
     7021 cgggatgtcg ggttcagcag caaacgacga aaactacgct gcacaccgat gatgcttaag
     7081 tcgaacagaa agtaatcgta tgcgaaacga tcctcatcct gtctcttgat cagatcttga
     7141 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     7201 cccaacctta ccagagggcg gcccaactgg caattccgac gtctgtgcaa gtactactgt
     7261 tctgcagtca cttgcctaag atactagctc cggcaaaaaa acgggcaagg tgtcaccacc
     7321 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga cgtctaagaa
     7381 aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccagt
     7441 gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaatttc ggccggcttg
     7501 tcgacgacgg cggtctccgt cgtcaggatc atccgggcaa gctttccaca cattataggt
     7561 acaaaaagat gcgaaagcaa ataaattttt tgggtttgta ccgtacacca ctgagaccgc
     7621 ggtggttgac cagacaaacc acgaagctgt caccggatgt gctttccggt ctgatgagtc
     7681 cgtgaggacg aaacagcctc tacaaataat tttgtttaag ggcccaagtt cacgagaacg
     7741 atatagaaag ttcaaagagg agaaatacta gatgcgtaaa ggcgaagagc tgttcactgg
     7801 tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg
     7861 tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac
     7921 tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg
     7981 ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga
     8041 aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc
     8101 ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt
     8161 taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt
     8221 ttacatcacc gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa
     8281 cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga
     8341 tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga
     8401 tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac
     8461 gcatggtatg gatgaactgt acaaatgatg agagagaaga ttttcagcct gatacagatt
     8521 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
     8581 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     8641 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     8701 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     8761 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     8821 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     8881 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc
     8941 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9001 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9061 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcagcaaact
     9121 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9181 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9241 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9301 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9361 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9421 agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg
     9481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     9541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     9601 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9661 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     9721 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9781 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9841 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     9901 caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9961 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10021 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10081 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10141 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
    10201 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt caggcatttg
    10261 agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat
    10321 aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt
    10381 tctgccattc atccgcttat tatcacttat tcaggcgtag caaccaggcg tttaagggca
    10441 ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
    10501 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
    10561 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
    10621 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
    10681 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
    10741 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
    10801 acaccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
    10861 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
    10921 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
    10981 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
    11041 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
    11101 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
    11161 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
    11221 atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc
    11281 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc
    11341 gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
    11401 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
    11461 gcaaaagcac cgccggacat cagcgctagc ggagtgtata ctggcttact atgttggcac
    11521 tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt
    11581 cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg
    11641 gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat
    11701 gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc
    11761 tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga
    11821 caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc
    11881 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
    11941 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
    12001 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
    12061 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
    12121 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
    12181 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
    12241 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaa
//
