LOCUS       sequence-493221-       14259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid was employed to generate the mouse model used in this 
            study. It contains the human HNF4A gene enhancer linked to the Shh 
            mini-promoter and the Gsta2-IRES2-H2BeGFP. Homologous arm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14259)
  AUTHORS   Cheng SY, Guo J, Simonian TL, Caldarelli P, McMahon AP
  TITLE     Enhanced Expression of the Female-Biased Gene Gsta2 in Male Proximal
            Tubule Cells Improves Renal Resilience to Ischemia-Reperfusion 
            Injury.
  JOURNAL   J Am Soc Nephrol. 2025 Aug 21. doi: 10.1681/ASN.0000000840.
  PUBMED    40839384
REFERENCE   2  (bases 1 to 14259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Soc 
            Nephrol. 2025 Aug 21. doi: 10.1681/ASN.0000000840."
COMMENT     Sequence Label: pHNF4A-Enh_Gsta2-IRES2-H2BeGFP
FEATURES             Location/Qualifiers
     source          1..14259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3412..3428
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3412..3428
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    3425..3559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3449..3468)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3503..3522
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(3580..4293,4294..4296,4297..4299))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3583..4293,4294..4296,4297..4299))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3625..3646)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3974..3993
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4233..4254
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4296..4305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4318..4695)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     misc_feature    join(4702..4710,4711..4713,4714..5288)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4875..4894)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5104..5121
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     regulatory      6027..6036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(11401..11420)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11402..11420)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11427..11444)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11427..11443)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11436..11458)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             11581..11883
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11762..11781
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             12232..13026
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             12232..13026
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12286..12305)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12896..12915
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(13276..14067,14068..14136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13899..13918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcat tgactgcagg ctgagtgagt
     1081 gggggtacgt aaggacccaa agctatgtag cctagcttct caaggtgtct aaagaaagga
     1141 agggtagatt accaatgagc agagtttggg cttctctatt tttaaagttt taaaatgttt
     1201 atatcaaggc aaggtttctg ttgtccttgc tggtctggaa ctagattggc cttgacctaa
     1261 gagatgatgc gacttctgcc tcccaagtgc taattaaagg tgtgagtcac cacgcttgcc
     1321 ttggtgtttt ctatttatag gttatatatt catttttcct attgcatgtc acttcccaaa
     1381 ttacatctca tctagtcatc tgtgcaccca atcacaaata ggcataacct ggtagatttc
     1441 cactaaaagt tacttagagg aagtttgtga atttattatt acaggcaagc catggaaatg
     1501 tgtgtagctg ttgcagttgg tttgaggagg tgtaagtgca tggtgtattt ggtagagaga
     1561 ctaggctgcc tagttcatta tgagaagata tgtacaacac aaacgaagta gggtctcagg
     1621 ttcaactgtt aagctttctt gcctcaggct gctggagagt tgcactcgta cttattcact
     1681 caattcattc tttggccagg tgggtggtgg tggtggtggt tcacaccttt agtcatagtg
     1741 ttgaggaggc agaggcaaga ggttctgtgt ttgaggccag ctgggtctat agattgaatt
     1801 ctaggaaagc cagggctaca tagagaaacc tgttttcaaa agaaaaaaga aaaaaagaaa
     1861 agacgaatgg tgtatgtgtg tgtttgtcag tcatctgtct ggaattgaat ttgagatccc
     1921 cctgcctccc aagtgtcctg attaaaggca tagtctatat agctgggagt ggtggcgcac
     1981 acttttaatt ctagcacttg agaggcagag gcaggcggat ctctgagttc gaggccagcc
     2041 tggtcaacaa agtgagttcc aggacagcca aggctacaca gagaaaccct gtctcgaaaa
     2101 aacccaaaaa aaaaaaaaaa aaaaagagta aaggcatagt ctaccacatc cactgaggtg
     2161 tgttcttaca gccaaagact ttttctcctg aggtctaagg gaacctcgta gtatgagtct
     2221 catgacatgg aatgaatttc ctgttttgtt ttcttggcct tagttaagct aagcagaccc
     2281 actattctgc accactcatt agttaggatg ctaacactca tttaagaggt taatgattga
     2341 aaaggcattt tcacagaaac catatggcgc tccagtggct ttcattaggt catttgagaa
     2401 tccagatttt ctcatcttct ctattcacta caggttgttg gcagttttgg ccagttagct
     2461 caccttgaaa atggaaacat gtctgacaag agccttgagc tgaatatcat ccagagtatt
     2521 tgctagagac agggtcttaa gtctcattaa attgcttaga agtgtgttta gtcctataaa
     2581 ctatgtctca tttgtgtgtc cttcacaaag agcgctagtg tcgatcatca attagcctag
     2641 cctataaagg tgacacaacc tggtcagtcc ctctgtatgt ctactatttc cccttctgat
     2701 ttctactatc ttctcaagaa ctggttcttc agcttccttt gggaaatgtc actttttaat
     2761 gatgtgtgtt ttgcttatgt atatgtctac atactaccca tgtgcttggt cactgcagag
     2821 gccagaagac ggtgtcaggt gcactggaac tagagttaat gacagatgtg agccataggg
     2881 tgctttgttc acatctttcc agtcccaaat gcgtctaaac ttatgtgata catactagat
     2941 taccacaaaa ttgctccaaa gacacctttc tccctctgag atgaatctct ggctggcctt
     3001 gctctaggca atcctgtgtt caattcaagg actgaatttg attacatcgg taatccagtg
     3061 ccccaggcat ttctgctttt tctgtaaggt tcttatcccc tggaagactg tttacgtatt
     3121 catttttttt tgagactaag tttcaggtag aaaaagcttg ccttgaactt cactatatag
     3181 ggcttatctt gaactcttgt gggtcttcca cctttcttca gttagcttct gtacactgcc
     3241 agacatgaaa atcagatcca tttatagata gatggtcatg agccaccatg tgggtgtcta
     3301 aaattgatct cgggacctct gaaagaccag ctagttctct taactgctga gccatctctc
     3361 tagcgtgtct atacacattt aatatcccct tgttcccttt ctgcttgccg ctctagaact
     3421 agtggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3481 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3541 aagctgcaat aaacaagtta acaacaactt aacaacaact tacttgtaca gctcgtccat
     3601 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     3661 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
     3721 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     3781 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
     3841 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     3901 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     3961 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     4021 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     4081 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
     4141 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     4201 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     4261 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcctt
     4321 agcgctggtg tacttggtga tggccttagt accctcggac acggcgtgct tggccaactc
     4381 cccaggcagc agcaggcgca cggccgtctg gatctccctg gaggtgatgg tcgagcgctt
     4441 gttgtaatgc gccaggcggg aagcctcacc tgcgatgcgc tcgaaaatgt cgttcacaaa
     4501 cgaattcatg atgcccatgg ccttggacga aatgccggtg tcagggtgga cctgcttcag
     4561 aaccttgtac acatagatgg aatagctctc cttgcggctg cgcttgcgct tcttgccgcc
     4621 tttcttctgc gccttagtca ccgccttctt ggagcccttt ttcggggcgg gagcagactt
     4681 cgctggctct ggcataacca tggttgtggc catattatca tcgtgttttt caaaggaaaa
     4741 ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa
     4801 agcatgtgta ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct
     4861 tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac
     4921 tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt
     4981 ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag
     5041 acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac
     5101 cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc
     5161 caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga
     5221 caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg gggggggagg
     5281 gagaggggcg gatcccccgg gctgcaggaa ttcgatatca accattggga tcggccagta
     5341 acgttagggg ggggggaggg actaaaactt gaaaaccttc cttgcttctt caatttgttt
     5401 tgcatccaag ggaggctttc tctggctgcc aggatgtagg aacttcttca cattggggag
     5461 gctgctgatt ctgctcttga aggccttcag cagagggaaa ggggtcagaa ggctggcatc
     5521 aagctcttca acatagagaa gaagttccag caggtggacg tccaccctgg tcagcctgtt
     5581 gcccacaagg tagtcttgtc catggctctt caacaccttt tcaaaggcag gcaagtaacg
     5641 gtttttggtc ctgtcttttg ccaaggcagt cttggcttct ctttggtctg ggggacataa
     5701 taccaattgc ccaatcattt cagtcagatc taaaatacct tctgtataca tgtcaatcag
     5761 ggctctctcc ttcatgtcct tcccatagag gtcatatttg gtggcgatgt agttgagaat
     5821 ggctctggtc tgcaccagct tcatcccatc aatctccacc atgggcactt ggtcaaacat
     5881 caaattccca tcttttttta gcttttccaa atcttccgga ctctgtataa acttctcttc
     5941 aaactccacc cctgctgcag ccaggagcca cctgatgcac tccattctgc cccgggcatt
     6001 gaagtagtga agcacgggct tcccggccat ggtggcatct cgtccgcgga acctgaggac
     6061 ttgtgagctg tccccgcgct gtctgtgcgc gagcgggtac gcgcgggtgt gtgcgtgtgc
     6121 gctcctcctt gcgctcggct ctcccttcct cgctcggctc gttcgttccc tctggcgctg
     6181 cctggctctt tctcttccta tataaccttg cctgccgctg ctgcagggga cttgtctccg
     6241 atccccaccc agactggggc tgcgatggca ggctgccggt cgctggtaac ggaacacatc
     6301 ggagttgggt ctcgagacag caattaaaag acaaaaagag cctgatttta aatggtagca
     6361 aggctggaga gcttgtgaga caggctgcct ttcgcactat attatagctg gcaggggcgc
     6421 atctgattgg ccaagccgca caagcttgtc ttctgatgtt aaccctgaga aagactacat
     6481 ttctcccccc tctggctgct gtggctttat tttcacctga aggcttccag tttaaaaaag
     6541 aaagaaagag agaaagaaag gctgctcagt ttggatgttc ctggcggcca atcccagttc
     6601 tgtcgctaac ttgctctgtg acttcaggca aatttctgtc tctctctaag ccccaatctc
     6661 tttctgcaat tgtatccagg cccaagaagc acaccaactg ggaatctagt ttctggggca
     6721 atgtcaacaa aattgggaag ccctttggag gacaatgccc ccgactttcc cagggatgcc
     6781 ccacctgcat tgggcaagct gggctcctca tataccagga atggggagct gggaaccctt
     6841 ctgatcccag gttcaacccc ctgatatggc agctgttctg gggggtgggc cgggaggtcg
     6901 gggtggtgtg ttaatgattg tcctgtgtta ttggccaccc atcattgctc tctgagaatg
     6961 ctctgagccc cagccaggga ggtttgggtg ggaaatcgag gttaacaatt gctcaggttg
     7021 ggggtatctt gttgccctgc taagcccctg ggaaggaaca ggagggatta aacattaatg
     7081 tagagactcc agcagagggg actggggcca gagcacaagg aacaggggag agggtagggg
     7141 cggattgtcc aagatccttg ccctgtcgac tctgctaatg gaaggaggaa ccatggaaga
     7201 gggccccggg tccagcccct gtatgaggct ggggacaagc caaaccagcg ttttcacagt
     7261 gtcccaatgt gcagtattaa taggtgttcc aggagaatct tgccgaagga gtttgagaag
     7321 cctcggcctc tgcaaagtca aacaactgcc tggacgagac ttctcagaag ctttcacaga
     7381 ctcacatgca ttgtcagtgt cttatgcaaa ggtgcgaaac gggtaagcga atgcaccaga
     7441 gtctgtctcc gaagctgaga agttgcaggg gaactcaagg acctgagaag taaagcacct
     7501 tatcccgggg tataggcagg ggcaggggca tcctccacac gcctggccac cccagggctg
     7561 cttggcatct tcacttcccc ttggcgctca ccacaccatt atctcctatc ttttcttccc
     7621 cacccaccac tccgggagag gtgcagagaa aactgggact tatcaagaca aagaacaaaa
     7681 gtcgtggagg aaagaagcca agagccatct ctactctggg gtagggccct tcagttttgc
     7741 ccctttgaaa tttcaaattc cagtttgtgg acaaagtcct aactatctca caatataggt
     7801 ccccaaccac tgaccaaact ccagtccagg cagccaccag ctggcctggt cttgctgctt
     7861 cctttagcgg cttccaaggt ccagggacag ggggtctggg ccaccaagag gctctgctag
     7921 gctgtccctg cagccacagc caccccactg gacccctgcc tcccatctga gaggacagcc
     7981 ctcttcctgg agctgggatc tgacacttgc tgataccaac ggcagatacc aggtaggtcc
     8041 cctccctttc tcttccttga gctcctaaaa tgccactcat accaccctct gcactcggca
     8101 aaggccactg tggcttaatc accatagact tggggcccag acctggggcc cctcacggag
     8161 cctcagaggg tgaaagggac ttgctcaagt ccacgcagcg gtggtgggtg agctttgtct
     8221 taacaacgag cactttctac ttcatcacag accatgagtg gcattgtgtt tgttcacccg
     8281 atgatgggtg gccactggct tgttcacccg atgatgggtg gccactggct tgtcacagga
     8341 gtatttccga gaggaagatg agggagcttg gggctgaagg ccagtgggtt tggaaggaag
     8401 caacattcat gtggttaact gataactggg ccccatccct gggcctcctt cctccctccc
     8461 tgtccttagc ctgattaaga ccaacttacc cagctgctaa tcattgctga gcctgttgat
     8521 tttgccttaa aaaattaact gggcatgagt ttgcggtttt cctcacccct aaccctgaaa
     8581 aggcctgggt gggcaggagg cctccagggt tatgcaagag gccgctggcc tcccaacgca
     8641 tagcccacgg ccacctcggc tgctctccaa agaagaggct ttgctcaaga gtcaacagtc
     8701 tgcttgttcc ccctgtgctg ggtcctgagc aaatttgctc tgttaccaaa gagagataaa
     8761 ggcagctggg ggagagcttt ccaaacaggg atgaccagag gcacagggag gttgagccat
     8821 atacccatgc tcacacagct gatgagaaac aagaagtgga attggaactt gggcagtctg
     8881 tctcaacagt ctgtgcctct aactccaggg ctacccttcc cttccaggga tgccagtgag
     8941 agatggccag gagaagggca gagagcaggg cactgtggga gatgctgact gtaaggctcc
     9001 ggaggtaggg cagagagtat ggtctggaga atgatcagga aggaagccag gaggacttgg
     9061 atctgtgtgg gtggagcttc tccgcaggct gcctcctgcc aggtggctta tgacatctgg
     9121 gctgagggtg ttgctgtcct cctgtttatg ttcatccctc ctgtttatgt tcctgaattt
     9181 atctgcaggg gtgaccttgg gaattagatg cggccttccc ggcccttgtg gggcctgaag
     9241 gctacctggg gatgggcagg acgatcccct gagctagtgg cctttgcctg cacaggtcat
     9301 tttagctcca atgagatgcc actttgcaaa agttctttga ggatcttaaa gggcacggtg
     9361 gaggctgcta aacttgagag aaggactctg aggagggcaa ggggcgcacc tgagggagtg
     9421 gggctgctaa gtctcacttg atagactcca cagttttgca acttggatct gggccttcac
     9481 aaaccttcgc gaacccgcag ccccacccct cgggtcagcc aatgtcttct gtggggctgg
     9541 gagccagggg aagaggccaa aggaactgtg cagagaagcg tggccacccg ggccgcagct
     9601 gggagccctg acaccctttg ccgccccacc tcatcccgcg gttgccccag ggcgcagggc
     9661 agggcaggca ccgcgctggg ccggagggcg tcccggagga ggcggccaag actctccgca
     9721 gtgctgcgct ttgcgcttcc tgggctcctc ctcgtggcca acgcaggaac tggtgttcag
     9781 aaacttagat agccttaggg acttcaccaa tcacagcaat cccgccaatc acaggcccag
     9841 acgcactatg tctctccaaa tcgtggcata tgtaactttt aataaaccaa aagaaaaagt
     9901 cccaaatatt caagtgaaaa aaatccaaac agttgacaca agcctaactg atagttactt
     9961 tatgtacagc tgtatgtata agttcaaaaa aagctaccct atttgtatgt acaaaagttt
    10021 atacacaggt ctgtacataa gggtctatac attttatttt ttcagaaccc ttaggtgtca
    10081 cctctagaag acaccaacac ttcattcaca tattttataa aagaaagact tccaggactg
    10141 acaatcttgt atcccttgta tttgaaccat gtaggttcat tcgatttaac agccttctgt
    10201 cataatgtag atcatggtag gtatgtggat cccctaaaca tcttcacata gtgtgctcca
    10261 atgtaaaagc ccattacaaa gagggaatga gagtcatgac cagggtgtca ccacaggtcc
    10321 aggtatgggt gagccagcct gccctcttca ctgtctatat tccagttcat ctacactgat
    10381 gactttggtg ggcatggagg gcagaggctg ctgtagcaca tctgagccaa accatttggc
    10441 aaggcccaca ggggagctgc tcctttggct ggtacgatgc tccagctggg agtgcatgtg
    10501 gggcatgccc gaccggctgg gtacattctg aggagtctga acgctgatag ctgctgcctg
    10561 ggaactagag cctggaggat gcagaactat ggagagaaga gtgtcagtgc agcaaagtag
    10621 ctctactcta cacctcaccc acagaacccc acaagtctac taagaccacc cttggatact
    10681 cactcccaag tcctgttctc agtattgaca atattttctg acctactttg cttgaatggt
    10741 ttgttacttt gggggatggg aagtctacca gaggatttaa ccaaaggctt cctaaagtgc
    10801 aaaatgcaac gtaagaaaat gcaacgtaag aaagcccacc aaggctgaca tgttcacatc
    10861 tcagcaaaaa ttctcaggct tataagaagt gaaaaacaga gctagagcat ggctggctcc
    10921 gactgtcctt gcagtagtaa taatccctga gactgcacag gtcagttcac ttcacctgtt
    10981 tttctagtga cacctaagaa acaaaggggc agtgataact attgctcctg gaaatctcag
    11041 aactttccag tgtgatcagc taacctggcc tatttctaaa ccttacctga gcgctgtagc
    11101 tgctgctgca tcaccgccaa atgcagaggg gtcccaggac gagggtttag gagagggtga
    11161 ctagcagcag agaccaaagg gtacaagggt tgacccagga gtgggccaga tagcccctgt
    11221 aaaggagcca tgtctatccc aggaggaagc acacctgatg gggagaaaga agaaactcat
    11281 gagttgtaga tgttcctaaa aatgtcaaag tttactactt ttgttgattt ggatacttac
    11341 aaggaggtgg atgtggtgaa cacttgccac catacatgta ccagggctaa attcaattcg
    11401 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
    11461 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    11521 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct atacgtacgg
    11581 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
    11641 agtgatatta ttgacacgcc ggggcgacgg atggtgatcc ccctggccag tgcacgtctg
    11701 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
    11761 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
    11821 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
    11881 taaatgtcag gcatgagatt atcaaaaagg atcttcacct agatcctttt cacgtagaaa
    11941 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca
    12001 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag
    12061 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct
    12121 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga
    12181 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa
    12241 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
    12301 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
    12361 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
    12421 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
    12481 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
    12541 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
    12601 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
    12661 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
    12721 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
    12781 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
    12841 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
    12901 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
    12961 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
    13021 ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
    13081 ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    13141 tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa aaaggatctt
    13201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    13261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    13321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    13381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    13441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    13501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    13561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    13621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    13681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    13741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    14041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    14101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    14161 aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt tttcgttcca
    14221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
