LOCUS       sequence-493224-        9473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of NLS-tagged EGFP from EF1-alpha promoter 
            (for lentiviral packaging).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Van Eyndhoven LC, Kinsler G, Zhang J, O'Farrell A, Abderrahim R, 
            Srivastav A, Triandafillou CG, Greco TM, Zaret KS, Cristea IM, 
            Orzalli MH, Drayman N, Singh A, Raj A
  TITLE     Lineage memory shapes viral resistance barriers in human skin.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 27:2025.06.27.661951. doi: 
            10.1101/2025.06.27.661951.
  PUBMED    40666908
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 27:2025.06.27.661951. doi: 
            10.1101/2025.06.27.661951."
COMMENT     Sequence Label: pCGT002_nuclear-EGFP
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    890..911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(926..931,932..949,950..956)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    964..980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     969..991
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     988..1004
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     988..1004
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1023..1043
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1025..1043
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1071..1297
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1298..1478
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1525..1650
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2143..2376
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2561..2605
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2836..3046
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2858..3054
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2905..3040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3076..4095
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    4166..4283
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4421..5599
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4652..5590
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5547..5567
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5607..5616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5619..5639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5637..5639,5640..6350)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5637..5639,5640..6350)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5679..5700)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5940..5959)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6287..6308
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6351..6371
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6396..6984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6449..6469)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6867..6878)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7056..7289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7367..7488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7404..7423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7458..7477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7528..7663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7683..7702)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7684..7702)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7712..7729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7712..7728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7721..7743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7870..8325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7957..7976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8167..8188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8351..8455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8456..8524,8525..9316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8674..8693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      841 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      901 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      961 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     1021 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     1081 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     1141 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     1201 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     1261 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     1321 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1381 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1441 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1501 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1561 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1621 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1681 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1741 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1801 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1861 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1921 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1981 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     2041 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     2101 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     2161 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     2221 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     2281 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     2341 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2401 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2461 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2521 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2581 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2641 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2701 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2761 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
     2821 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
     2881 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2941 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3001 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     3061 tctagaggta ccaccatgaa aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg
     3121 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct ccgagggcga agaatctcgg
     3181 gctttcagct tcgatgtggg agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat
     3241 ggtttctaca aagatcgcta tgtttatcgg cactttgcat ccgccgctct ccctattccc
     3301 gaagtgcttg acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca
     3361 cagggtgtca ccttgcaaga cctgcctgaa accgaactgc ccgctgttct ccagcccgtc
     3421 gccgaggcca tggatgccat cgctgccgcc gatcttagcc agaccagcgg gttcggccca
     3481 ttcggacctc aaggaatcgg tcaatacact acatggcgcg atttcatctg cgctattgct
     3541 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc
     3601 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     3661 cacgccgatt tcggctccaa caatgtcctg accgacaatg gccgcataac agccgtcatt
     3721 gactggagcg aggccatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     3781 aggccctggt tggcttgtat ggagcagcag acccgctact tcgagcggag gcatcccgag
     3841 cttgcaggat ctcctcggct ccgggcttat atgctccgca ttggtcttga ccaactctat
     3901 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca
     3961 atcgtccggt ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc
     4021 gtctggaccg atggctgtgt ggaagtgctc gccgatagtg gaaacagacg ccccagcact
     4081 cgtcctaggg caaaggatct gcagtaatga ggcgcgccgt taacgaattc tagatcttga
     4141 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     4201 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     4261 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     4321 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     4381 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
     4441 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     4501 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     4561 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     4621 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     4681 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     4741 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     4801 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     4861 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     4921 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     4981 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     5041 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     5101 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     5161 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     5221 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     5281 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     5341 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     5401 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     5461 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     5521 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     5581 tccatttcag gtgtcgtgag gctagcgcca ccatggtgcc taagaaaaaa cggaaagtga
     5641 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5701 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     5761 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5821 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     5881 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     5941 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     6001 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6061 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     6121 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     6181 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6241 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6301 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag cctaagaaaa
     6361 aacggaaagt atgaggatcc acgcgttaag tcgacaatca acctctggat tacaaaattt
     6421 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6481 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6541 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6601 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6661 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6721 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6781 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6841 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6901 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6961 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     7021 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7081 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7261 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     7321 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7501 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7561 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7621 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     7681 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     7741 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7801 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7861 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7921 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7981 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8041 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8101 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8161 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8221 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8281 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     8341 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8401 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8461 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8521 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8581 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8641 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8701 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8761 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8821 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8881 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8941 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9001 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9061 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9121 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9181 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9241 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9301 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9361 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9421 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa aga
//
