LOCUS       sequence-49323-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, 
            Tsien RY
  TITLE     Ca2+ indicators based on computationally redesigned 
            calmodulin-peptide pairs.
  JOURNAL   Chem Biol. 2006 May;13(5):521-30.
  PUBMED    16720273
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2006-05"; volume: "13(5)"; pages: "521-30"
COMMENT     Sequence Label: pBAD-D4
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(139..651,652..654,655..657))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     332..351
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     591..612
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg
       61 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      121 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      181 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      241 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      301 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      361 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      421 catgtggtcg gggtagcggg cgaagcacat caggccgtag ccgaaggtgg tcacgagggt
      481 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
      541 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
      601 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatgag
      661 ctccagtgcc ccggagctgg agatcttctt cagtcttcct
//
