LOCUS       sequence-49324-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: PIGAZFN-R1
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcaccatgg gacctaagaa aaagaggaag gtggcggccg
      121 ctgactacaa ggatgacgac gataaatcta gacccgggga gcgccccttc cagtgtcgca
      181 tttgcatgcg gaacttttcg gcccccagca agcttaagag gcatacccgt actcataccg
      241 gtgaaaaacc gtttcagtgt cggatctgta tgcgaaattt ctcccacaag agcagcttga
      301 cgcggcatct acgtacgcac accggcgaga agccattcca atgccgaata tgcatgcgca
      361 acttcagtca gcggaacgcg ctggccgggc acctaaaaac ccacctgagg ggatcccaac
      421 tagtcaaaag tgaactggag gagaagaaat ctgaacttcg tcataaattg aaatatgtgc
      481 ctcatgaata tattgaatta attgaaattg ccagaaattc cactcaggat agaattcttg
      541 aaatgaaggt aatggaattt tttatgaaag tttatggata tagaggtaaa catttgggtg
      601 gatcaaggaa accggacgga gcaatttata ctgtcggatc tcctattgat tacggtgtga
      661 tcgtggatac taaagcttat agcggaggtt ataatctgcc aattggccaa gcagatgaaa
      721 tgcaacgata tgtcgaagaa aatcaaacac gaaacaaaca tatcaaccct aatgaatggt
      781 ggaaagtcta tccatcttct gtaacggaat ttaagttttt atttgtgagt ggtcacttta
      841 aaggaaacta caaagctcag cttacacgat taaatcatat cactaattgt aatg
//
