LOCUS       sequence-49326-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Palmer AE, Jin C, Reed JC, Tsien RY
  TITLE     Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed 
            with an improved genetically encoded fluorescent sensor.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Epub 2004 Dec
            7.
  PUBMED    15585581
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-12-14"; volume: "101(50)"; pages: 
            "17404-9. Epub 2004 Dec 7"
COMMENT     Sequence Label: pcDNA-D1
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(70..780,781..783,784..786))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(112..133)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     461..480
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     720..741
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcttac ttgtagagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      121 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actggtagct
      181 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      241 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cgaatcttga agttcacctt
      301 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
      361 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      421 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
      481 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
      541 gtgctgcttc atgtggtcgg ggtagcgggc gaagcacatc aggccgtagc cgaaggtggt
      601 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
      661 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
      721 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
      781 caccatgagc tccagtgccc cggagctgga gatcttctcg aactcgttgg cagcgctg
//
