LOCUS       sequence-49327-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: PIGAZFN-R1
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             116..703
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             719..760
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             770..787
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcggcatcta cgtacgcaca ccggcgagaa gccattccaa tgccgaatat gcatgcgcaa
       61 cttcagtcag cggaacgcgc tggccgggca cctaaaaacc cacctgaggg gatcccaact
      121 agtcaaaagt gaactggagg agaagaaatc tgaacttcgt cataaattga aatatgtgcc
      181 tcatgaatat attgaattaa ttgaaattgc cagaaattcc actcaggata gaattcttga
      241 aatgaaggta atggaatttt ttatgaaagt ttatggatat agaggtaaac atttgggtgg
      301 atcaaggaaa ccggacggag caatttatac tgtcggatct cctattgatt acggtgtgat
      361 cgtggatact aaagcttata gcggaggtta taatctgcca attggccaag cagatgaaat
      421 gcaacgatat gtcgaagaaa atcaaacacg aaacaaacat atcaacccta atgaatggtg
      481 gaaagtctat ccatcttctg taacggaatt taagttttta tttgtgagtg gtcactttaa
      541 aggaaactac aaagctcagc ttacacgatt aaatcatatc actaattgta atggagctgt
      601 tcttagtgta gaagagcttt taattggtgg agaaatgatt aaagccggca cattaacctt
      661 agaggaagtg agacggaaat ttaataacgg cgagataaac ttttaagggc ccttcgaagg
      721 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
      781 tcaccattga gtac
//
