LOCUS       sequence-49330-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: PIGAZFN-R2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             125..148
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcaccat gggacctaag aaaaagagga aggtggcggc
      121 cgctgactac aaggatgacg acgataaatc tagacccggg gagcgcccct tccagtgtcg
      181 catttgcatg cggaactttt cggcccccag caagcttaag aggcataccc gtactcatac
      241 cggtgaaaaa ccgtttcagt gtcggatctg tatgcgaaat ttctcccaca agagcagctt
      301 gacgcggcat ctacgtacgc acaccggcga gaagccattc caatgccgaa tatgcatgcg
      361 caacttcagt cagcggaacg cgctgagcgg gcacctaaaa acccacctga ggggatccca
      421 actagtcaaa agtgaactgg aggagaagaa atctgaactt cgtcataaat tgaaatatgt
      481 gcctcatgaa tatattgaat taattgaaat tgccagaaat tccactcagg atagaattct
      541 tgaaatgaag gtaatggaat tttttatgaa agtttatgga tatagaggta aacatttggg
      601 tggatcaagg aaaccggacg gagcaattta tactgtcgga tctcctattg attacggtgt
      661 gatcgtggat actaaagctt atagcggagg ttataatctg ccaattggcc aagcagatga
      721 aatgcaacga tatgtcgaag aaaatcaaac acgaaacaaa catatcaacc ctaatgaatg
      781 gtggaaagtc tatccatctt ctgtaacgga atttaagttt ttatttgtga gtggtcactt
      841 taaaggaaac
//
