LOCUS       sequence-49331-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: PIGAZFN-R2
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..650
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             666..707
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             717..734
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgcgcaactt cagtcagcgg aacgcgctga gcgggcacct aaaaacccac ctgaggggat
       61 cccaactagt caaaagtgaa ctggaggaga agaaatctga acttcgtcat aaattgaaat
      121 atgtgcctca tgaatatatt gaattaattg aaattgccag aaattccact caggatagaa
      181 ttcttgaaat gaaggtaatg gaatttttta tgaaagttta tggatataga ggtaaacatt
      241 tgggtggatc aaggaaaccg gacggagcaa tttatactgt cggatctcct attgattacg
      301 gtgtgatcgt ggatactaaa gcttatagcg gaggttataa tctgccaatt ggccaagcag
      361 atgaaatgca acgatatgtc gaagaaaatc aaacacgaaa caaacatatc aaccctaatg
      421 aatggtggaa agtctatcca tcttctgtaa cggaatttaa gtttttattt gtgagtggtc
      481 actttaaagg aaactacaaa gctcagctta cacgattaaa tcatatcact aattgtaatg
      541 gagctgttct tagtgtagaa gagcttttaa ttggtggaga aatgattaaa gccggcacat
      601 taaccttaga ggaagtgaga cggaaattta ataacggcga gataaacttt taagggccct
      661 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc
      721 atcaccatca ccattgagt
//
