LOCUS       sequence-49332-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan 
            EK
  TITLE     Divergent GW182 functional domains in the regulation of 
            translational silencing.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
  PUBMED    21131274
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(7)"; pages: "2534-47. Epub
            2010 Dec 2"
COMMENT     Sequence Label: NHA-Delta 5a
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..117
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    175..199
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(535..559)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    612..733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(623..642)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     677..696
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 caaccgacga gtgagcgtcg cgctgagaaa caagctcaat ggaaagctgc aaacccaccg
       61 ctcgaggntg cccaagctta caccatggcc tacccatatg atgttccaga ttacgcttca
      121 ctcgacgcgg atcccgtcga attcacgcgt ggtacctcta gagtcgaccc atcaacaagt
      181 ttgtacaaaa aagcaggctc ctcatccttg aacaccacgc tgccttcaac tagtgcctgg
      241 tcatccattc gtgcctccaa ctacaacgtt cccctcagca gtacagcaca aagcacttca
      301 gccagaaata gtgattccaa attgacatgg tctcctggtt cagttacaaa cacctctctg
      361 gctcatgagc tgtggaaggt ccctttgcca cctaaaaaca tcactgctcc gtcccgccca
      421 cctccgggac tgactggtca gaagccaccc ttgtctacgt gggataattc tccccttcgt
      481 ataggtggag gatggggaaa ttctgacgcc agatataccc caggttccag ctgaacccag
      541 ctttcttgta caaagtggtt gatgggcggc cgcttccctt tagtgagggt taatgcttcg
      601 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      661 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      721 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
      781 gtgggaggtt tttttaaagc aagtaaaaac ctctacaatg tggtaaaatc cgataaggat
      841 cgatccgggc tggggtaata
//
