LOCUS       sequence-49336-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Gierke S, Wittmann T
  TITLE     EB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion 
            Dynamics during 3D Epithelial Remodeling.
  JOURNAL   Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5.
  PUBMED    22483942
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2012-05-8"; volume: "22(9)"; pages: "753-62. Epub 
            2012 Apr 5"
COMMENT     Sequence Label: pLKO.1 EB1 shRNA #1
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    126..243
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        292..802
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     721..739
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 gctttatata tcttgtggaa ggacgaaaca ccgggtgaaa ttccaggcta aacctcgagg
       61 tttagcctgg aatttcactt ttttgaattc tcgacctcga gacaaatggc agtattcatc
      121 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      181 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      241 tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg
      301 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
      361 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
      421 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
      481 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
      541 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
      601 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
      661 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
      721 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
      781 aatcaccgac ctctctcccc agggggatcc accggagctt accatg
//
