LOCUS       sequence-493398-        9384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression lentiviral vector encoding the wild-type 
            Synaptobrevin2 (Syb2) with R56X mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B,
            Brunger AT, Kavalali ET
  TITLE     Synaptobrevin-2 disease variants reveal spatial constraints within 
            the presynaptic active zone.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 
            10.1073/pnas.2507347122. Epub 2025 Oct 30.
  PUBMED    41166419
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-11-4"; volume: "122(44):e2507347122. 
            doi"; pages: " 10.1073/pnas.2507347122. Epub 2025 Oct 30"
COMMENT     Sequence Label: pFUW-Syb2-R56X
FEATURES             Location/Qualifiers
     source          1..9384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2621..3832
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3669..3688
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    4075..4663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4128..4148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4546..4557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4666..4682)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4667..4683)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5188..5368
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5394..5411)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5400..5624
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5670..6098
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5757..5776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5967..5988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6107..6127)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6112..6441
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6292..6427
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6354..6373
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6489..6536
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6555..6929
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7059..7180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7096..7115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7150..7169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7229..7245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7229..7245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7242..7264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7253..7269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7277..7283,7284..7301,7302..7307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7322..7343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7460..7477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7631..8219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7711..7730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8390..9181,9182..9250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9013..9032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9251..9355)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg
     2641 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc
     2701 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac
     2761 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct
     2821 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc
     2881 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg
     2941 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat
     3001 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg
     3061 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc
     3121 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc
     3181 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg
     3241 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct
     3301 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga
     3361 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca
     3421 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt
     3481 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg
     3541 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg
     3601 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct
     3661 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga
     3721 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
     3781 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
     3841 ggctgcaggt cgactctaga ggatcgaatt catgtcggct accgctgcca ccgtcccgcc
     3901 tgccgccccg gccggcgagg gtggcccccc tgcacctcct ccaaatctta ccagtaacag
     3961 gagactgcag cagacccagg cccaggtgga tgaggtggtg gacatcatga gggtgaatgt
     4021 ggacaaggtc ctggagtaag gatccgttaa caattcgata tcaagcttat cgataatcaa
     4081 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4141 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4201 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4261 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4321 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4381 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4441 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4501 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4561 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4621 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     4681 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     4741 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     4801 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4861 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     4921 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     4981 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     5041 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     5101 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     5161 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5221 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5281 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5341 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     5401 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5461 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5521 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5581 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5641 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5701 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5761 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5821 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5881 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5941 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6001 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6061 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6121 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6181 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6241 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6301 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6361 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6421 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6481 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     6541 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     6601 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     6661 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     6721 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     6781 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     6841 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     6901 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     6961 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7021 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7201 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7261 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7321 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7381 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7441 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7501 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7561 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7621 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7801 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8041 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8281 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8341 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8401 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8461 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8521 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8581 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8641 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8701 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8761 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8821 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8881 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8941 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9001 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9061 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9121 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9181 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9241 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9361 ttccccgaaa agtgccacct gacg
//
