LOCUS       sequence-49342-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAG-YFP
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(254..273)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             413..664
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     601..622
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
       61 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      121 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca
      181 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      241 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      301 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      361 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      421 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      481 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      541 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      601 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      661 caagtaaagc ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
      721 ggccaatgcc ctggctcaca aataccactg agatcttt
//
