LOCUS       sequence-49346-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAG-YFP
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(88..107)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    153..208
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(207..226)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(569..585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(569..585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(582..604)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(617..623,624..641,642..647))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    662..683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
       61 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
      121 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
      181 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
      241 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
      301 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
      361 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
      421 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
      481 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
      541 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      601 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      661 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      721 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcc
//
