LOCUS       sequence-49355-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Koivunen P, Lee S, Duncan CG, Lopez G, Lu G, Ramkissoon S, Losman 
            JA, Joensuu P, Bergmann U, Gross S, Travins J, Weiss S, Looper R, 
            Ligon KL, Verhaak RG, Yan H, Kaelin WG Jr
  TITLE     Transformation by the (R)-enantiomer of 2-hydroxyglutarate linked to
            EGLN activation.
  JOURNAL   Nature. 2012 Feb 15;483(7390):484-8. doi: 10.1038/nature10898.
  PUBMED    22343896
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-02-15- 15"; volume: "483"; issue: "7390"; 
            pages: "484-8"
COMMENT     Sequence Label: FLAG-EglN2-pLenti6
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttttgacctc catagaagac accgactcta gccaccatgg actacaaaga cgatgacgat
       61 aaagggccct ctagactcga catggacagc ccgtgccagc cgcagcccct aagtcaggct
      121 ctccctcagt taccagggtc ttcgtcagag cccttggagc ctgagcctgg ccgggccagg
      181 atgggagtgg agagttacct gccctgtccc ctgctcccct cctaccactg tccaggagtg
      241 cctagtgagg cctcggcagg gagtgggacc cccagagcca cagccacctc taccactgcc
      301 agccctcttc gggacggttt tggcgggcag gatggtggtg agctgcggcc gctgcagagt
      361 gaaggcgctg cagcgctggt caccaagggg tgccagcgat tggcagccca gggcgcacgg
      421 cctgaggccc ccaaacggaa atgggccgag gatggtgggg atgccccttc acccagcaaa
      481 cggccctggg ccaggcaaga gaaccaggag gcagagcggg agggtggcat gagctgcagc
      541 tgcagcagtg gcagtggtga ggccagtgct gggctgatgg aggaggcgct gccctctgcg
      601 cccgagcgcc tggccctgga ctatatcgtg ccctgcatgc ggtactacgg catctgcgtc
      661 aaggacagct tcctgggggc agcactgggc ggtcgcgtgc tggccgaggt
//
