LOCUS       sequence-49357-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Koivunen P, Lee S, Duncan CG, Lopez G, Lu G, Ramkissoon S, Losman 
            JA, Joensuu P, Bergmann U, Gross S, Travins J, Weiss S, Looper R, 
            Ligon KL, Verhaak RG, Yan H, Kaelin WG Jr
  TITLE     Transformation by the (R)-enantiomer of 2-hydroxyglutarate linked to
            EGLN activation.
  JOURNAL   Nature. 2012 Feb 15;483(7390):484-8. doi: 10.1038/nature10898.
  PUBMED    22343896
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-02-15- 15"; volume: "483"; issue: "7390"; 
            pages: "484-8"
COMMENT     Sequence Label: FLAG-EglN3-pLenti6
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(845..865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ccgctgtttt gacctccata gaagacaccg actctagcca ccatggacta caaagacgat
       61 gacgataaag ggccctctag actcgacatg cccctgggac acatcatgag gctggacctg
      121 gagaaaattg ccctggagta catcgtgccc tgtctgcacg aggtgggctt ctgctacctg
      181 gacaacttcc tgggcgaggt ggtgggcgac tgcgtcctgg agcgcgtcaa gcagctgcac
      241 tgcaccgggg ccctgcggga cggccagctg gcggggccgc gcgccggcgt ctccaagcga
      301 cacctgcggg gcgaccagat cacgtggatc gggggcaacg aggagggctg cgaggccatc
      361 agcttcctcc tgtccctcat cgacaggctg gtcctctact gcgggagccg gctgggcaaa
      421 tactacgtca aggagaggtc taaggcaatg gtggcttgct atccgggaaa tggaacaggt
      481 tatgttcgcc acgtggacaa ccccaacggt gatggtcgct gcatcacctg catctactat
      541 ctgaacaaga attgggatgc caagctacat ggtgggatcc tgcggatatt tccagagggg
      601 aaatcattca tagcagatgt ggagcccatt tttgacagac tcctgttctt ctggtcagat
      661 cgtaggaacc cacacgaagt gcagccctct tacgcaacca gatatgctat gactgtctgg
      721 tactttgatg ctgaagaaag ggcagaagcc aaaaagaaat tcaggaattt aactaggaaa
      781 actgaatctg ccctcactga agactgagaa ttccggttag taatgagttt ggaattaatt
      841 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
      901 atgcaaagca tgcatctcaa
//
