LOCUS       sequence-49360-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Koivunen P, Lee S, Duncan CG, Lopez G, Lu G, Ramkissoon S, Losman 
            JA, Joensuu P, Bergmann U, Gross S, Travins J, Weiss S, Looper R, 
            Ligon KL, Verhaak RG, Yan H, Kaelin WG Jr
  TITLE     Transformation by the (R)-enantiomer of 2-hydroxyglutarate linked to
            EGLN activation.
  JOURNAL   Nature. 2012 Feb 15;483(7390):484-8. doi: 10.1038/nature10898.
  PUBMED    22343896
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-02-15- 15"; volume: "483"; issue: "7390"; 
            pages: "484-8"
COMMENT     Sequence Label: FLAG-EglN1-pLenti6
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccgact ctagccacca tggactacaa agacgatgac gataaagggc
      121 cctctagact cgacatggcc aatgacagcg gcgggcccgg cgggccgagc ccgagcgagc
      181 gagaccggca gtactgcgag ctgtgcggga agatggagaa cctgctgcgc tgcagccgct
      241 gccgcagctc cttctactgc tgcaaggagc accagcgtca ggactggaag aagcacaagc
      301 tcgtgtgcca gggcagcgag ggcgccctcg gccacggagt gggcccacac cagcattccg
      361 gccccgcgcc gccggctgca gtgccgccgc ccagggccgg ggcccgggag cccaggaagg
      421 cagcggcgcg ccgggacaac gcctccgggg acgcggccaa gggaaaagta aaggccaagc
      481 ccccggccga cccagcggcg gccgcgtcgc cgtgtcgtgc ggccgccggc ggccagggct
      541 cggcggtggc tgccgaagcc gagcccggca aggaggagcc gccggcccgc tcatcgctgt
      601 tccaggagaa ggcgaacctg taccccccaa gcaacacgcc cggggatgcg ctgagccccg
      661 gcggcggcct gcggcccaac gggcagacga agcccctgcc ggcgctgaag ctggcgctcg
      721 agtacatcgt gccgtgcatg aacaagcacg gcatctgtgt ggtggacgac ttcctcggca
      781 aggagaccgg acagcagatc ggcgacgagg tgcgcgccct gcacgacacc gggaagttca
      841 cggacgggca gctggtcagc cagaagagtg actcgtccaa ggacatccga ggcgataag
//
