LOCUS       sequence-49361-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Gierke S, Wittmann T
  TITLE     EB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion 
            Dynamics during 3D Epithelial Remodeling.
  JOURNAL   Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5.
  PUBMED    22483942
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2012-05-8"; volume: "22(9)"; pages: "753-62. Epub 
            2012 Apr 5"
COMMENT     Sequence Label: pEB1-2xEGFP
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(5..7,8..10,11..724)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5..7,8..10,11..721)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(50..71)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(311..330)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     658..679
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
       61 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      121 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      181 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      241 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      301 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      361 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      421 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      481 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      541 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      601 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      661 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      721 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
      781 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      841 gttgttaact tgtttattgc agcttat
//
