LOCUS       sequence-49368-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Gierke S, Wittmann T
  TITLE     EB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion 
            Dynamics during 3D Epithelial Remodeling.
  JOURNAL   Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5.
  PUBMED    22483942
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2012-05-8"; volume: "22(9)"; pages: "753-62. Epub 
            2012 Apr 5"
COMMENT     Sequence Label: pEB1deltaC-2xEGFP
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(137..850,851..853))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(140..850,851..853))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(182..203)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     531..550
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     790..811
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctgcataac agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
       61 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      121 tagagtcgcg gccgctttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      181 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
      241 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      301 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
      361 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
      421 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      481 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      541 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      601 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
      661 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
      721 tcagggtcag cttgccgtaa gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
      781 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
      841 cgcccttgct cacgatggtg gcgaccggtg tcttgtacag ctcgtccatg c
//
