LOCUS       sequence-493696-        9803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IVT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Datlinger P, Pankevich EV, Arnold CD, Pranckevicius N, Lin J, 
            Romanovskaia D, Schaefer M, Piras F, Orts AC, Nemc A, Biesaga PN, 
            Chan M, Neuwirth T, Artemov AV, Li W, Ladstatter S, Krausgruber T, 
            Bock C
  TITLE     Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
  JOURNAL   Nature. 2025 Oct;646(8086):963-972. doi: 10.1038/s41586-025-09507-9.
            Epub 2025 Sep 24.
  PUBMED    40993398
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09507-9"; journalName: "Nature"; date: 
            "2025-10"; volume: "646"; issue: "8086"; pages: "963-972"
COMMENT     Sequence Label: pCART0002_pIVTRup(BsmBI)_CBE4max-SpRY
FEATURES             Location/Qualifiers
     source          1..9803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..59
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     51..68
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     52..68
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        165..183
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             265..285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             286..969
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             1066..5166
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5197..5445
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5476..5724
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5767..5787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6109..6125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6109..6125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6122..6144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6133..6149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6157..6163,6164..6181,6182..6187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6202..6223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6340..6357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6511..7099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6591..6610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7270..8061,8062..8130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7893..7912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(8148..8942)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8259..8278)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8869..8888
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(9286..9714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9396..9417)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9608..9627
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
       61 cggccagtga attgtaatac gactcactat agggcgaatt gggccctcta gatgcatgct
      121 cgagcggccg ccagtgtgat ggatttggac cctcgtacag aagctaatac gactcactat
      181 agggaaataa gagagaaaag aagagtaaga agaaatataa gagccaccat gaaacggaca
      241 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctcctc agagactggg
      301 cctgtcgccg tcgatccaac cctgcgccgc cggattgaac ctcacgagtt tgaagtgttc
      361 tttgaccccc gggagctgag aaaggagaca tgcctgctgt acgagatcaa ctggggaggc
      421 aggcactcca tctggaggca cacctctcag aacacaaata agcacgtgga ggtgaacttc
      481 atcgagaagt ttaccacaga gcggtacttc tgccccaata ccagatgtag catcacatgg
      541 tttctgagct ggtccccttg cggagagtgt agcagggcca tcaccgagtt cctgtccaga
      601 tatccacacg tgacactgtt tatctacatc gccaggctgt atcaccacgc agacccaagg
      661 aataggcagg gcctgcgcga tctgatcagc tccggcgtga ccatccagat catgacagag
      721 caggagtccg gctactgctg gcggaacttc gtgaattatt ctcctagcaa cgaggcccac
      781 tggcctaggt acccacacct gtgggtgcgc ctgtacgtgc tggagctgta ttgcatcatc
      841 ctgggcctgc ccccttgtct gaatatcctg cggagaaagc agccccagct gaccttcttt
      901 acaatcgccc tgcagtcttg tcactatcag aggctgccac cccacatcct gtgggccaca
      961 ggcctgaagt ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc
     1021 gagtcagcaa caccagagag cagtggcggc agcagcggcg gcagcgacaa gaagtacagc
     1081 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1141 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1201 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg agagaacccg gctgaagaga
     1261 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     1321 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     1381 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     1441 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     1501 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     1561 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     1621 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     1681 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     1741 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     1801 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     1861 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     1921 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     1981 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2041 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2101 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2161 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2221 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     2281 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     2341 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     2401 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     2461 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     2521 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     2581 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     2641 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     2701 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     2761 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     2821 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     2881 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     2941 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3001 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3061 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3121 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3181 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3241 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     3301 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     3361 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     3421 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     3481 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     3541 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     3601 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     3661 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     3721 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     3781 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     3841 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     3901 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     3961 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4021 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4081 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4141 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4201 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     4261 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     4321 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     4381 aaagagtcta tcagacccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     4441 gaccctaaga agtacggcgg cttcctgtgg cccaccgtgg cctattctgt gctggtggtg
     4501 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     4561 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     4621 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     4681 gaaaacggcc ggaagagaat gctggcctct gccaagcagc tgcagaaggg aaacgaactg
     4741 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     4801 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     4861 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     4921 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     4981 gagaatatca tccacctgtt taccctgacc agactgggag cccctagagc cttcaagtac
     5041 tttgacacca ccatcgaccc caagcagtac agaagcacca aagaggtgct ggacgccacc
     5101 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5161 ggtgacagcg gcgggagcgg cgggagcggg gggagcacta atctgagcga catcattgag
     5221 aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg
     5281 gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag
     5341 tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc
     5401 ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgagcgg aggatccgga
     5461 ggatctggag gcagcaccaa cctgtctgac atcatcgaga aggagacagg caagcagctg
     5521 gtcatccagg agagcatcct gatgctgccc gaagaagtcg aagaagtgat cggaaacaag
     5581 cctgagagcg atatcctggt ccataccgcc tacgacgaga gtaccgacga aaatgtgatg
     5641 ctgctgacat ccgacgcccc agagtataag ccctgggctc tggtcatcca ggattccaac
     5701 ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa
     5761 ttcgagccca agaagaagag gaaagtcgga ggcggttgaa tcgctgcctt ctgcggggct
     5821 tgccttctgg ccatgccctt cttctctccc ttgcacctgt acctcttggt ctttgaataa
     5881 agcctgagta ggaagtgagg gtctagaaaa aaaaaaaaaa aaaaaaaaag aaaaaaaaaa
     5941 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaatga gacgcgatca
     6001 ataaccgaca ctcctgcgtc gcctacgcgc tacgatctgc agaattccag cacactggcg
     6061 gccgttacta gtggatccga gctcggtacc aagcttggcg taatcatggt catagctgtt
     6121 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6181 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6241 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6301 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7261 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7321 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7381 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7441 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7501 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7561 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7621 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7681 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7741 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7801 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7861 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7921 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7981 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8041 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8101 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaattca gaagaactcg
     8161 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     8221 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     8281 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     8341 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     8401 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     8461 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     8521 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     8581 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtggga tgacaggaga
     8641 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     8701 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
     8761 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     8821 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     8881 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     8941 atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc
     9001 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
     9061 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
     9121 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
     9181 atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac
     9241 gtgttccgct tcctttagca gcccttgcgc cctgaatttt gttaaaattc gcgttaaatt
     9301 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     9361 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     9421 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     9481 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     9541 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     9601 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     9661 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     9721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     9781 ccagctggcg aaagggggat gtg
//
