LOCUS       sequence-49373-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Callaway lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-HB
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..67)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(118..151)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgaggtaccg gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg
       61 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata
      121 acttcgtata gcatacatta tacgaagtta tggcgcgccc tattacttgt acatcttaca
      181 gtctggtctc acccccactc ttgtgtgatt cccatgaaga tatgatcttc ccgctttggg
      241 gagtgactga cacctccctc cctgtccctc tgagattgtg ttgcgtaggt tctgatcgat
      301 tgactcttct acaacatgtc atcaggaaaa ttatcaacat caaggcagtc agggcccctg
      361 cactcagtaa tacatacttc ccccagttcg ggagacccaa gtcaactcct gagacctgat
      421 tgtgcacatc gggaaggtga acttcaacaa aatcctcagc ctcgtcaccg tccttgaaaa
      481 cggtagacgg gtctgccagg gggtgcacaa gggggataac cgaggattcc aacaactcca
      541 tatgttgctg gaggagggat gattgcatct ctgggattaa gacattgccg tcaggtccta
      601 atattatacc attgaaaaac accccgttca catgaggatg acacctcccc ccaactctta
      661 aacacccttt tgaagggagg atctcattcc aagttctgac tgacttgtag tgagcatcgg
      721 cttccatcaa ggtcttgttg aatatggtat atgcttttcc aaacccaggg acaagttttc
      781 ttaaatgact gagacgtctg
//
