LOCUS       sequence-49374-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     Callaway lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-HB
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..86
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 gagctgtaca gggtagtggt gtgaaacaga ctttgaattt tgaccttctc aagttggcgg
       61 gagacgtgga gtccaaccca gggcccatgg ttcctcaggc tctcctgttt gtaccccttc
      121 tggtttttcc attgtgtttt gggaaattcc ctatttacac gataccagac aagcttggtc
      181 cctggagtcc gattgacata catcacctca gctgcccaaa caatttggta gtggaggacg
      241 aaggatgcac caacctgtca gggttctcct acatggaact taaagttgga tacatcttag
      301 ccataaaagt gaacgggttc acttgcacag gcgttgtgac ggaggctgaa acctacacta
      361 acttcgttgg ttatgtcaca accacgttca aaagaaagca tttccgccca acaccagatg
      421 catgtagagc cgcgtacaac tggaagatgg ccggtgaccc cagatatgaa gagtctctac
      481 acaatccgta ccctgactac cgctggcttc gaactgtaaa aaccaccaag gagtctctcg
      541 ttatcatatc tccaagtgtg gcagatttgg acccatatga cagatccctt cactcgaggg
      601 tcttccctag cgggaagtgc tcaggagtag cggtgtcttc tacctactgc tccactaacc
      661 acgattacac catttggatg cccgagaatc cgagactagg gatgtcttgt gacattttta
      721 ccaatagtag agggaagaga gcatccaaag ggagtgagac ttgcggcttt gtagatgaaa
//
