LOCUS       sequence-49375-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Miyazaki Y, Imoto H, Chen LC, Wandless TJ
  TITLE     Destabilizing domains derived from the human estrogen receptor.
  JOURNAL   J Am Chem Soc. 2012 Mar 7;134(9):3942-5. Epub 2012 Feb 22.
  PUBMED    22332638
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2012-03-7"; volume: "134(9)"; pages: "3942-5. Epub 
            2012 Feb 22"
COMMENT     Sequence Label: PBMN YFP-ER50
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    10..384
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     322..338
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             402..428
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(474..495)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(735..754)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcccattggc catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac
       61 cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac
      121 ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca actggaccga
      181 ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg acaccagact
      241 aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac ccccaccgcc
      301 ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc tgccgacccc
      361 gggggtggac catcctctag actgccggat ccgccaccat gtatccgtac gacgtaccgg
      421 attatgcgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      481 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      541 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      601 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
      661 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      721 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      781 acaccctggt gaaccgcatc gagctgaagg ct
//
