LOCUS       sequence-493811-        6169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PodJ-Delta-CC4-6 under the control of the PodJ 
            promoter (KanR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Lu N, Duvall SW, Zhao G, Kowallis KA, Zhang C, Tan W, Sun J, 
            Petitjean HN, Tomares DT, Zhao GP, Childers WS, Zhao W
  TITLE     Scaffold-Scaffold Interaction Facilitates Cell Polarity Development 
            in Caulobacter crescentus.
  JOURNAL   mBio. 2023 Apr 25;14(2):e0321822. doi: 10.1128/mbio.03218-22. Epub 
            2023 Mar 27.
  PUBMED    36971555
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2023-04-25"; volume: "14(2):e0321822. doi"; pages: " 
            10.1128/mbio.03218-22. Epub 2023 Mar 27"
COMMENT     Sequence Label: pLN055
FEATURES             Location/Qualifiers
     source          1..6169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            21..130
                     /note="incP origin of transfer"
     primer_bind     282..298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      299..345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1118..1135
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     primer_bind     complement(3993..4009)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4020..4036)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4029..4051)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4234..4822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4314..4333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5232..6047)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5232..6047)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5955..5974
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6039..6058
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 gtgcggcgcc gaccatgatg gacgagctga agtccatctc ggactcctgg ctttcgcatc
      541 acgccggcgg cgaaaagagc ttctcgatgg ggggctttga cccacgttat gtggccgagt
      601 tccccgtcgc ggttgttcgc agtgaaggca agatcgtcgc cttcgccacg ctgtggacga
      661 cggccgccaa gacggcgttc tccatggacc tgatgcgcta ctctgacgaa gcgcccaaga
      721 acgtcatgga ctatctgttc gtcgagctgc tgcagtgggg caaggacgag ggatacagcg
      781 ccttcgagtt cggcgtcgcg ccgctggcgg gtctgcagga tcgccctctg gccccgatca
      841 tgtcgcgcgt cggccggctg ctctacgagc gcggcgagga gatctataac ttccagggtg
      901 tgcgtcgcta caaggacaag tacgacccgg tctggcagcc gcgctacatt gccgcgcctc
      961 agaagtgggc gatcccgttc ctgctcgccg acatcgggct gctgtcgtcc ggtggggtct
     1021 ccggcctggc gaaacgaccc aagaagctcc cggagaaggc cgcgacctag tagcgcgtct
     1081 ggttacccgt ctggacgccg agcaggttca ccagatgcac cagcatcagc atcagtccga
     1141 ccacgcagat catcatcatc ggccgccaag ggatcagccg cgggcccttg agaatattga
     1201 tcggacgcgc gcccagccag ccgaagagca cggcgaggat caggaacagg atcgcggcgg
     1261 cggcggtgac gggcaggtcc atgcgcgctt ctaggcctgc gacaatccgc ctggcaaatg
     1321 gttaaccagt gttaaccctc ttgcttttga accgcgctac agagtgatgg gactctgtcc
     1381 tgattcctcc acagtgaatc gcacagccac tacatctggc gttttcgtcg cgtttacacc
     1441 ctaggaatcg gtgactagcg ttttccccag gtgcggggag atcgattcgc atgacggcgg
     1501 cttcgccatg gagcgttaag gggattgacc ccaaggcacg ggaggtcgcg aaagacctcg
     1561 cgcgtcgctc cggcatgact ttgggtgaat ggctgaaccg aatgatcatc gaaggcgatg
     1621 gtcagaccgc tgatccgcgt ctcgcggggg acgatgtccc caaccgcgcc tatctcgaga
     1681 ttgtcaaaga tgatgcgccg ccgcgcatcg agatcgccga gcatccggca gatgaggttg
     1741 gtcgcgtcgc gctggcgctg gatcgcctga cccaacgcat tgaagccgcc gaggggcgca
     1801 acgccgccgc catcaccggc atcgatcact ccgtccgtga cgccctgacg cgtctgggcg
     1861 catccgaacg tgagcagatc gccgtcgccg cccggttcga gggcgctgtc gacgaactga
     1921 agaccgagca ggctcgggcc accgaacgtc tgcgccggat cgagagcgag gcggcgggtc
     1981 cgcgctcggc cgaggcgttg cgcgcgcttg aaggcgcctt gggcaaggtc gccggacacc
     2041 tttacgaagg cgaggcgcgg acgcgcgagg cgatcgcgac cctggaagcc aagctgaacc
     2101 agcagagctc gggcgatccc agcgcgctgg tcgaggctgt ggtcgcgcga ctgggcgaac
     2161 gcctcgaagc cgccgaaacc cgcaccagcg acgcgctgcg cgaactgggc gcgtcgttcc
     2221 aggccctgga tcagcgccag gaactggtcg accgcatccg ccagagcgaa gagcgcacgc
     2281 tccgcatgct ggaagaagcg cgcgagaaga tcgacagccg tctgtccgag gcgcagcgga
     2341 agctggaagc tgcgccgccg tcgccgccgc ccgcgcaggc tccggcgccg gttgcgactg
     2401 ctcagcgtcc ggttccgccg gcggcttcgc ccttcgagga caactatttc agccaggccg
     2461 cctcgttcag cacgtctgag gatgaggctg acgcgtttga tgcgccgcct gcgccggccc
     2521 gtagcttcga ggtggcggag ttcccggctg cggagccgga agaaccggcc ttcgcacacg
     2581 atgactacgc gatcgccgat ggcttcgagc cggaaagccc gcgctatgaa gtcgagcctg
     2641 aggtctccga cttcgcgccc gccgagccgt cgcgtccgat gtcgacccgc gatatcatcg
     2701 agcaggcccg cgccgccgcg cgcgccgccg ccgcctcgga aggcaagggc ggcaaggcca
     2761 agtcggcgaa gaaggaaaag gcttccaagg ccagcggctc gctgttcagc ggctttggcg
     2821 gtttctcgac caagaagtcg aaggcgcgct tgggcgcgac cgtgacgacg gccctcgttg
     2881 tcttcgccgc cgccggcgcg ctcggggccg gcgtgggcgg cctgctgctg ctgaacaccg
     2941 acgacggcaa caactcgccc agccgcgtcg cccaggcgat cgctggtcgc aaggccgatg
     3001 tcgaggtgaa tggtcccgag gctgacacga cccccggcgc gccgcgcgcc gccgtcgcgc
     3061 tgacgacggg caaggtcgtc ccagccgagg tcgaggcccc tgcagccccg ccgaccaacg
     3121 aagccaaggc cctgttcgag gacgcggtcc gtaagatcga gtcgggggac cgctcgggcg
     3181 tcgagttgct gaagcgcgcc gccaatggcg gctacccggc cgcgcagttc tacctctcga
     3241 agatgtacga gggcggcaag aacggcgtga aggtcgatat ggccgaagcg cgccgctgga
     3301 gtgagcgcgc cgccaacggt ggcgatccgc gggccatgca caacctggcc ctctactact
     3361 tcaagggcga gggcggtccg cgcaattcga cgacggccgc cagctggttc cgcaaggccg
     3421 ccgacatggg cctggtcgac agccagttca acctggctca gctctacgaa agcggactgg
     3481 gcgtcagcca gaatccggcc gaagcctaca aatggtatgt gatcgccggc cgcgctgggg
     3541 attcgaccgc gcggggccgc gccacggcgc tgcgctcgca gctgaccgca gaggcccagc
     3601 agaccgctga ccgctcggcc ttggccttcc gtccgcagac tcaggtccag accgccagtc
     3661 tgtcgtccgc tgcgccggcc gccgcaaacg ccaaccttgg cgttgcgcag cgtgtcctgt
     3721 cgcagctggg ctattaccag gggccgcgcg acggtgtctc gtcgcccgcc ctgcgcatgg
     3781 cgatcgcggc ctatcagcgt gatcagggtc tgccccccac cggatcggtg gacgccgaga
     3841 ccctcaatcg cctgtcggtc tacgcgcgct aaacgcctcg cgacctcgcg ctttgccggg
     3901 aaggggcctt aattaatatg catggtacct taagatctcg agctccggag aattcgaacg
     3961 ttacgcgtac cggtgctagc tgcagcccgg gggatccact agttctagag cggccattca
     4021 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4081 cttgcagcac atcccccttt cgccagcggt atcagctcac tcaaaggcgg taatacggtt
     4141 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4201 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4261 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4681 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4741 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4861 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4921 ctagatcctt ttaggggggg gggggcgctg aggtctgcct cgtgaagaag gtgttgctga
     4981 ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc cacggttgat
     5041 gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg ccacggaacg
     5101 gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgatttat
     5161 tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta
     5221 accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     5281 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     5341 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     5401 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     5461 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     5521 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     5581 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     5641 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     5701 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     5761 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     5821 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     5881 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     5941 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     6001 tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     6061 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     6121 atgtaacatc agagattttg agacacaacg tggctttccc ccccccccc
//
