LOCUS       sequence-493812-        6199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PodJ-Delta-IDR under the control of the PodJ promoter 
            (KanR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Lu N, Duvall SW, Zhao G, Kowallis KA, Zhang C, Tan W, Sun J, 
            Petitjean HN, Tomares DT, Zhao GP, Childers WS, Zhao W
  TITLE     Scaffold-Scaffold Interaction Facilitates Cell Polarity Development 
            in Caulobacter crescentus.
  JOURNAL   mBio. 2023 Apr 25;14(2):e0321822. doi: 10.1128/mbio.03218-22. Epub 
            2023 Mar 27.
  PUBMED    36971555
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2023-04-25"; volume: "14(2):e0321822. doi"; pages: " 
            10.1128/mbio.03218-22. Epub 2023 Mar 27"
COMMENT     Sequence Label: pLN058
FEATURES             Location/Qualifiers
     source          1..6199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            21..130
                     /note="incP origin of transfer"
     primer_bind     282..298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      299..345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1118..1135
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     primer_bind     complement(4023..4039)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4050..4066)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4059..4081)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4264..4852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4344..4363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5262..6077)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5262..6077)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5985..6004
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6069..6088
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 gtgcggcgcc gaccatgatg gacgagctga agtccatctc ggactcctgg ctttcgcatc
      541 acgccggcgg cgaaaagagc ttctcgatgg ggggctttga cccacgttat gtggccgagt
      601 tccccgtcgc ggttgttcgc agtgaaggca agatcgtcgc cttcgccacg ctgtggacga
      661 cggccgccaa gacggcgttc tccatggacc tgatgcgcta ctctgacgaa gcgcccaaga
      721 acgtcatgga ctatctgttc gtcgagctgc tgcagtgggg caaggacgag ggatacagcg
      781 ccttcgagtt cggcgtcgcg ccgctggcgg gtctgcagga tcgccctctg gccccgatca
      841 tgtcgcgcgt cggccggctg ctctacgagc gcggcgagga gatctataac ttccagggtg
      901 tgcgtcgcta caaggacaag tacgacccgg tctggcagcc gcgctacatt gccgcgcctc
      961 agaagtgggc gatcccgttc ctgctcgccg acatcgggct gctgtcgtcc ggtggggtct
     1021 ccggcctggc gaaacgaccc aagaagctcc cggagaaggc cgcgacctag tagcgcgtct
     1081 ggttacccgt ctggacgccg agcaggttca ccagatgcac cagcatcagc atcagtccga
     1141 ccacgcagat catcatcatc ggccgccaag ggatcagccg cgggcccttg agaatattga
     1201 tcggacgcgc gcccagccag ccgaagagca cggcgaggat caggaacagg atcgcggcgg
     1261 cggcggtgac gggcaggtcc atgcgcgctt ctaggcctgc gacaatccgc ctggcaaatg
     1321 gttaaccagt gttaaccctc ttgcttttga accgcgctac agagtgatgg gactctgtcc
     1381 tgattcctcc acagtgaatc gcacagccac tacatctggc gttttcgtcg cgtttacacc
     1441 ctaggaatcg gtgactagcg ttttccccag gtgcggggag atcgattcgc atgacggcgg
     1501 cttcgccatg gagcgttaag gggattgacc ccaaggcacg ggaggtcgcg aaagacctcg
     1561 cgcgtcgctc cggcatgact ttgggtgaat ggctgaaccg aatgatcatc gaaggcgatg
     1621 gtcagaccgc tgatccgcgt ctcgcggggg acgatgtccc caaccgcgcc tatctcgaga
     1681 ttgtcaaaga tgatgcgccg ccgcgcatcg agatcgccga gcatccggca gatgaggttg
     1741 gtcgcgtcgc gctggcgctg gatcgcctga cccaacgcat tgaagccgcc gaggggcgca
     1801 acgccgccgc catcaccggc atcgatcact ccgtccgtga cgccctgacg cgtctgggcg
     1861 catccgaacg tgagcagatc gccgtcgccg cccggttcga gggcgctgtc gacgaactga
     1921 agaccgagca ggctcgggcc accgaacgtc tgcgccggat cgagagcgag gcggcgggtc
     1981 cgcgctcggc cgaggcgttg cgcgcgcttg aaggcgcctt gggcaaggtc gccggacacc
     2041 tttacgaagg cgaggcgcgg acgcgcgagg cgatcgcgac cctggaagcc aagctgaacc
     2101 agcagagctc gggcgatccc agcgcgctgg tcgaggctgt ggtcgcgcga ctgggcgaac
     2161 gcctcgaagc cgccgaaacc cgcaccagcg acgcgctgcg cgaactgggc gcgtcgttcc
     2221 aggccctgga tcagcgcttg ggcgccgtcg agactgccaa tcccgccacg ggcgttcagg
     2281 agggcctgga cagcctggcc gccaccctca cgcagaagat ggaagccgct cgcctggaga
     2341 tggcggccaa gcttcgcgaa agcgccgacg gacgtttcga tcgcatggag cgcaagctcg
     2401 gcgagatggc ggcccacgtg caggcggccg aacagcgctc ggcccaggcc attgagcgta
     2461 tgggccgcga gattgtcggt gtcgctgatg cgttcaatcg ccgcgtccac gccgccgaga
     2521 gccgtaacgc cagcgctatc gagcaggtcg gtggcgaggt tgcgcggatc gccgccagcg
     2581 tcgagcacaa gctcaatcgc gccgatagcg ttcaagccca ggcccttgaa aagctgggcg
     2641 gcgagatcgc ccggatcacc gagaagctgg ccgagcggat cggcagcgcc gagcgacgca
     2701 acgccctggc catcgacgat gtcggcgaac aggtcgcccg cgtcacagag cgcctgaacc
     2761 aacgccacga acgttccagc ggcaaggcca agtcggcgaa gaaggaaaag gcttccaagg
     2821 ccagcggctc gctgttcagc ggctttggcg gtttctcgac caagaagtcg aaggcgcgct
     2881 tgggcgcgac cgtgacgacg gccctcgttg tcttcgccgc cgccggcgcg ctcggggccg
     2941 gcgtgggcgg cctgctgctg ctgaacaccg acgacggcaa caactcgccc agccgcgtcg
     3001 cccaggcgat cgctggtcgc aaggccgatg tcgaggtgaa tggtcccgag gctgacacga
     3061 cccccggcgc gccgcgcgcc gccgtcgcgc tgacgacggg caaggtcgtc ccagccgagg
     3121 tcgaggcccc tgcagccccg ccgaccaacg aagccaaggc cctgttcgag gacgcggtcc
     3181 gtaagatcga gtcgggggac cgctcgggcg tcgagttgct gaagcgcgcc gccaatggcg
     3241 gctacccggc cgcgcagttc tacctctcga agatgtacga gggcggcaag aacggcgtga
     3301 aggtcgatat ggccgaagcg cgccgctgga gtgagcgcgc cgccaacggt ggcgatccgc
     3361 gggccatgca caacctggcc ctctactact tcaagggcga gggcggtccg cgcaattcga
     3421 cgacggccgc cagctggttc cgcaaggccg ccgacatggg cctggtcgac agccagttca
     3481 acctggctca gctctacgaa agcggactgg gcgtcagcca gaatccggcc gaagcctaca
     3541 aatggtatgt gatcgccggc cgcgctgggg attcgaccgc gcggggccgc gccacggcgc
     3601 tgcgctcgca gctgaccgca gaggcccagc agaccgctga ccgctcggcc ttggccttcc
     3661 gtccgcagac tcaggtccag accgccagtc tgtcgtccgc tgcgccggcc gccgcaaacg
     3721 ccaaccttgg cgttgcgcag cgtgtcctgt cgcagctggg ctattaccag gggccgcgcg
     3781 acggtgtctc gtcgcccgcc ctgcgcatgg cgatcgcggc ctatcagcgt gatcagggtc
     3841 tgccccccac cggatcggtg gacgccgaga ccctcaatcg cctgtcggtc tacgcgcgct
     3901 aaacgcctcg cgacctcgcg ctttgccggg aaggggcctt aattaatatg catggtacct
     3961 taagatctcg agctccggag aattcgaacg ttacgcgtac cggtgctagc tgcagcccgg
     4021 gggatccact agttctagag cggccattca ctggccgtcg ttttacaacg tcgtgactgg
     4081 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagcggt
     4141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg tgttgctggc
     4261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaggggggg gggggcgctg
     4981 aggtctgcct cgtgaagaag gtgttgctga ctcataccag gcctgaatcg ccccatcatc
     5041 cagccagaaa gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt
     5101 gattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg
     5161 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc
     5221 gtaatgctct gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc
     5281 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     5341 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     5401 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     5461 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     5521 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     5581 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     5641 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     5701 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     5761 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     5821 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     5881 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     5941 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     6001 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc
     6061 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     6121 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     6181 tggctttccc ccccccccc
//
