LOCUS       sequence-49382-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Suzuki T, Arai S, Takeuchi M, Sakurai C, Ebana H, Higashi T, 
            Hashimoto H, Hatsuzawa K, Wada I
  TITLE     Development of cysteine-free fluorescent proteins for the oxidative 
            environment.
  JOURNAL   PLoS One. 2012;7(5):e37551. Epub 2012 May 23.
  PUBMED    22649538
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(5)"; pages: "e37551. Epub 2012 May 23"
COMMENT     Sequence Label: ss-cfSGFP2
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      205..214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(211..213,214..216,217..729)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQMFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(256..277)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(517..536)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     864..885
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctacaatg
       61 ccgtcttctg tctcgtgggg catcctcctg ctggcaggcc tgtgctgcct ggtccctgtc
      121 tccctggctg aggatcccca gggagatgct gcccagaaga gaattctgca gtcgacggta
      181 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      241 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      301 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctccacc
      361 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      421 atgttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      481 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      541 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      601 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      661 gtctatatca cggccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
      721 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      781 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa actgagcaaa
      841 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      901 actctcggca
//
