LOCUS       sequence-493850-        3509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-dependent AAV expressing light-activatable FGFR1 (optoFGFR1-HA) 
            for temporally precise control of neuronal FGFR1 signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Shin J, Oh H, Park JH, Bae H, Yang CM, Lee M, Kim S, Heo WD
  TITLE     Dysregulation of FGFR1 signaling in the hippocampus facilitates 
            depressive disorder.
  JOURNAL   Exp Mol Med. 2025 Aug;57(8):1818-1836. doi: 
            10.1038/s12276-025-01519-9. Epub 2025 Aug 15.
  PUBMED    40813470
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s12276-025-01519-9"; journalName: "Exp Mol Med"; date: 
            "2025-08"; volume: "57"; issue: "8"; pages: "1818-1836"
COMMENT     Sequence Label: AAV-hSyn-DIO-optoFGFR1-HA
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    complement(467..500)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(551..584)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(606..632)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc tagagtcgac tccggaataa cttcgtatag
      481 gatactttat acgaagttat gcagaatggt agctggattg tagctgctat tagcaatatg
      541 aaacctctta ataacttcgt atagcataca ttatacgaag ttatgggcgg ccgcggcgcg
      601 ccttaagcat aatctggaac atcatatgga tataccggtg ggctgccgct gccggcagca
      661 ccgatcataa tctgcgcctc ccgagtgcga gagatcgctt tagccagcag ctcgcgtgct
      721 gtatctatat caactatagg cttagcgtaa ttggtgccca gctccacccc gcttgccttg
      781 agcactgtca gtggtgcgtc ccaaggatgg tgaatccact ccgtaggaag tcgggccagc
      841 tcggggagcc actgtcgaat atactctcct tcgggatcat acttagctcc ctgcaaggcc
      901 ggattgtcca acctgtccaa ctcatggccg tcaggaattg acccgctaat atattgccat
      961 cccagaatgt cacactccaa gtcggcgtcg agaagggtat cccaaaagta cttcattccc
     1021 catttccaag gcaggagcag aaacttgaca gcaaatgagg acacgattac ccggatgcgg
     1081 ttgtgcatcc agcctgtagc ccagagctct ctcatgccag catccacgag tgggtaccct
     1141 gtccttccct gtctccatgc cttgaatttg tcgacatcgg cgtcccaggg gaagaacctg
     1201 aggtgggaca acagactctg ctcgtgtgtg aatggaaagt tgaaacatat ataccgggag
     1261 tattcccgaa gtccgatccc tcgcaggaac agatcggcgc tttcggcgcc ttcgctgttt
     1321 ttatcgcgag cccagataat ctgtttcatc cgagcgcact gaaatacatg gcggacagaa
     1381 atctcgccaa aatggagata tggagagagc aagctagttg agttacctac cactttcttg
     1441 gagttcttcg cgtagtcaat cagttgtttt tcgatgaact cgttaagcag cttgtcggca
     1501 ttagaccagc cggggctcca ggctctagtg agaagtgcat tgcttggctt ttctgcttca
     1561 ttttccagac cgagttcctc gatggagcag gcccagatgg cctctgcagc agcagtaatc
     1621 ggcatcagtc tccagggagg gggcaacata acggactcta ttgacatatc caaacatttc
     1681 ttccagtaac tgttgaagga ggtgaacggt ttccctttct cgcaatagat ctcccatggc
     1741 tcgtacagaa ggtccccgtt gtagctctgc acactgatcc cccgttccac cagcttctcc
     1801 ttcacagtgt ggtcgcgcac cagactaact ggatcgtaca ggtggttaaa cacgactttc
     1861 gttgcgccgg tcaccctgat gcagtcgagg atggcagaga tggtattgtg tgtcttgatc
     1921 agggtaagat cactgcccag tgctttcagt gactggctaa gatgggccag actttgcttc
     1981 atccaccacc tagaggccct tccgggataa aactggcctt cttcttcagg acaccaaatg
     2041 aaaacgggga agacggaacc ctcgtgggcc gcggcggcga gcgcgggatt atcttctatt
     2101 ctcaaatctc tccgaaacca gacgatggtc tttttgtcca tcttcatggg atccgcgcgg
     2161 cgtttgagtc cgccattggc aagctgggct gggtgtcggg gcaggcaggg ctcctcgggc
     2221 agcggctcat gagagaagac ggaatcctcc cctgaggagc acgtagagct ccgggtgtcg
     2281 ggaaagctgg gggagtactg gtccaggggc atggacaggt ccaggtactc ctggttggag
     2341 gtcaaggcca cgatgcggtc caggtcttcc accagctgct tgaaggtggg tctctgtgag
     2401 ggcactgcat gccagcagtc ccgcatcatc atgtacagct cgttggtgca gttactgggc
     2461 ttgtccatgc ggtgaccctc cttcagcagc ttgaaaagtt cctccacagg cacaccgggg
     2521 tatggggagc cgcccagagt gaagatctcc cacaggagca ccccgaaaga ccacacatca
     2581 ctctggtggg tgtagatccg gtcaaataat gcctcgggtg ccatccactt cacaggcagt
     2641 cggccgttgg ttgtcttttt atagtagtcg atgtggtgaa tgtcccgtgc gaggccaaag
     2701 tctgctatct tcatcacatt gtcctctgtc accaggacat tcctggctgc caggtctcgg
     2761 tgtatgcact tcttggaggc cagatactcc atgcctcggg ccacctggta ggcgcaggac
     2821 accaggtcct tggaggagag ctgctcctct gggttgtggc tggggttgta gcagtattcc
     2881 agccctgggg gcctccgggc ctgcaggtac tcccgcaggt tgcccttgga ggcatactcc
     2941 acgatgacat acaagggacc atcctgcgtg caggccccca gcaggttgat gatattctta
     3001 tgcttcccga tcatcttcat catctccatt tctgagatca ggtctgacaa gtctttctct
     3061 gttgcgtccg acttcaacat cttcacagcc actttggtca cacggttggg tttgtccttg
     3121 tccagcccga tagcctctgc caacaccacc tgcccaaagc agccctctcc caggggtttg
     3181 cctaagacca gtctgtcccg aggcagctcc cagcgagggt cttcgggaag ctcatactca
     3241 gagacccctg ctagcatggg agtcccactg gaggagagcc gtgatggccg aaccagaaga
     3301 accccagagt tcatggatgc actggagtca gcagacactg ttacctgtct gcgcagaggg
     3361 atgctcttgg ccagcttgtg cacagccatc tggctgtgga agtcactctt cttggtacca
     3421 ctcttcatct tggaattccc agcactacca gcactaccag cactatctgc tcccgcgctg
     3481 tctttccctt ttgattttat acatcccat
//
