LOCUS       sequence-493852-        3304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expressing light-activatable FGFR1 (optoFGFR1-HA) under the 
            CaMKII-alpha promoter for excitatory-neuron-specific activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Shin J, Oh H, Park JH, Bae H, Yang CM, Lee M, Kim S, Heo WD
  TITLE     Dysregulation of FGFR1 signaling in the hippocampus facilitates 
            depressive disorder.
  JOURNAL   Exp Mol Med. 2025 Aug;57(8):1818-1836. doi: 
            10.1038/s12276-025-01519-9. Epub 2025 Aug 15.
  PUBMED    40813470
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s12276-025-01519-9"; journalName: "Exp Mol Med"; date: 
            "2025-08"; volume: "57"; issue: "8"; pages: "1818-1836"
COMMENT     Sequence Label: AAV-CaMKII-alpha-optoFGFR1-HA
FEATURES             Location/Qualifiers
     source          1..3304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3278..3304
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 acttgtggac taagtttgtt cacatcccct tctccaaccc cctcagtaca tcaccctggg
       61 ggaacagggt ccacttgctc ctgggcccac acagtcctgc agtattgtgt atataaggcc
      121 agggcaaaga ggagcaggtt ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc
      181 ggggcaacgg gaacagggcg tttcggaggt ggttgccatg gggacctgga tgctgacgaa
      241 ggctcgcgag gctgtgagca gccacagtgc cctgctcaga agccccaagc tcgtcagtca
      301 agccggttct ccgtttgcac tcaggagcac gggcaggcga gtggccccta gttctggggg
      361 cagctctaga gccgatcccc gggtaccggt gctagccacc atgggatgta taaaatcaaa
      421 agggaaagac agcgcgggag cagatagtgc tggtagtgct ggtagtgctg ggaattccaa
      481 gatgaagagt ggtaccaaga agagtgactt ccacagccag atggctgtgc acaagctggc
      541 caagagcatc cctctgcgca gacaggtaac agtgtctgct gactccagtg catccatgaa
      601 ctctggggtt cttctggttc ggccatcacg gctctcctcc agtgggactc ccatgctagc
      661 aggggtctct gagtatgagc ttcccgaaga ccctcgctgg gagctgcctc gggacagact
      721 ggtcttaggc aaacccctgg gagagggctg ctttgggcag gtggtgttgg cagaggctat
      781 cgggctggac aaggacaaac ccaaccgtgt gaccaaagtg gctgtgaaga tgttgaagtc
      841 ggacgcaaca gagaaagact tgtcagacct gatctcagaa atggagatga tgaagatgat
      901 cgggaagcat aagaatatca tcaacctgct gggggcctgc acgcaggatg gtcccttgta
      961 tgtcatcgtg gagtatgcct ccaagggcaa cctgcgggag tacctgcagg cccggaggcc
     1021 cccagggctg gaatactgct acaaccccag ccacaaccca gaggagcagc tctcctccaa
     1081 ggacctggtg tcctgcgcct accaggtggc ccgaggcatg gagtatctgg cctccaagaa
     1141 gtgcatacac cgagacctgg cagccaggaa tgtcctggtg acagaggaca atgtgatgaa
     1201 gatagcagac tttggcctcg cacgggacat tcaccacatc gactactata aaaagacaac
     1261 caacggccga ctgcctgtga agtggatggc acccgaggca ttatttgacc ggatctacac
     1321 ccaccagagt gatgtgtggt ctttcggggt gctcctgtgg gagatcttca ctctgggcgg
     1381 ctccccatac cccggtgtgc ctgtggagga acttttcaag ctgctgaagg agggtcaccg
     1441 catggacaag cccagtaact gcaccaacga gctgtacatg atgatgcggg actgctggca
     1501 tgcagtgccc tcacagagac ccaccttcaa gcagctggtg gaagacctgg accgcatcgt
     1561 ggccttgacc tccaaccagg agtacctgga cctgtccatg cccctggacc agtactcccc
     1621 cagctttccc gacacccgga gctctacgtg ctcctcaggg gaggattccg tcttctctca
     1681 tgagccgctg cccgaggagc cctgcctgcc ccgacaccca gcccagcttg ccaatggcgg
     1741 actcaaacgc cgcgcggatc ccatgaagat ggacaaaaag accatcgtct ggtttcggag
     1801 agatttgaga atagaagata atcccgcgct cgccgccgcg gcccacgagg gttccgtctt
     1861 ccccgttttc atttggtgtc ctgaagaaga aggccagttt tatcccggaa gggcctctag
     1921 gtggtggatg aagcaaagtc tggcccatct tagccagtca ctgaaagcac tgggcagtga
     1981 tcttaccctg atcaagacac acaataccat ctctgccatc ctcgactgca tcagggtgac
     2041 cggcgcaacg aaagtcgtgt ttaaccacct gtacgatcca gttagtctgg tgcgcgacca
     2101 cactgtgaag gagaagctgg tggaacgggg gatcagtgtg cagagctaca acggggacct
     2161 tctgtacgag ccatgggaga tctattgcga gaaagggaaa ccgttcacct ccttcaacag
     2221 ttactggaag aaatgtttgg atatgtcaat agagtccgtt atgttgcccc ctccctggag
     2281 actgatgccg attactgctg ctgcagaggc catctgggcc tgctccatcg aggaactcgg
     2341 tctggaaaat gaagcagaaa agccaagcaa tgcacttctc actagagcct ggagccccgg
     2401 ctggtctaat gccgacaagc tgcttaacga gttcatcgaa aaacaactga ttgactacgc
     2461 gaagaactcc aagaaagtgg taggtaactc aactagcttg ctctctccat atctccattt
     2521 tggcgagatt tctgtccgcc atgtatttca gtgcgctcgg atgaaacaga ttatctgggc
     2581 tcgcgataaa aacagcgaag gcgccgaaag cgccgatctg ttcctgcgag ggatcggact
     2641 tcgggaatac tcccggtata tatgtttcaa ctttccattc acacacgagc agagtctgtt
     2701 gtcccacctc aggttcttcc cctgggacgc cgatgtcgac aaattcaagg catggagaca
     2761 gggaaggaca gggtacccac tcgtggatgc tggcatgaga gagctctggg ctacaggctg
     2821 gatgcacaac cgcatccggg taatcgtgtc ctcatttgct gtcaagtttc tgctcctgcc
     2881 ttggaaatgg ggaatgaagt acttttggga tacccttctc gacgccgact tggagtgtga
     2941 cattctggga tggcaatata ttagcgggtc aattcctgac ggccatgagt tggacaggtt
     3001 ggacaatccg gccttgcagg gagctaagta tgatcccgaa ggagagtata ttcgacagtg
     3061 gctccccgag ctggcccgac ttcctacgga gtggattcac catccttggg acgcaccact
     3121 gacagtgctc aaggcaagcg gggtggagct gggcaccaat tacgctaagc ctatagttga
     3181 tatagataca gcacgcgagc tgctggctaa agcgatctct cgcactcggg aggcgcagat
     3241 tatgatcggt gctgccggca gcggcagccc accggtatat ccatatgatg ttccagatta
     3301 tgct
//
