LOCUS       sequence-493899-        6641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human alpha-Synuclein A53T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6641)
  TITLE     GeneDetect Michael J Fox Foundation Shared Tools Plasmids 2022
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..6641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     promoter        1287..1564
                     /label=chicken beta-actin promoter
     primer_bind     1843..1865
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1927..1946
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2418..2444
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2464..3052
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2517..2537)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2935..2946)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3079..3096)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3085..3309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3519..3848)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3533..3668
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3833..3853
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4094..4682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4174..4193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4853..5644,5645..5713))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5476..5495
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5714..5818)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5886..5904
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5942..5964)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6064..6083
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6297..6316
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        6606..6624
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6606..6623
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggtacg atccccagct tgcatgcctg caggtcactg ttctcatcac atcatatcaa
      241 ggttatatac catcaatatt gccacagatg ttacttagcc ttttaatatt tctctaattt
      301 agtgtatatg caatgatagt tctctgattt ctgagattga gtttctcatg tgtaatgatt
      361 atttagagtt tctctttcat ctgttcaaat ttttgtctag ttttattttt tactgatttg
      421 taagacttct ttttataatc tgcatattac aattctcttt actggggtgt tgcaaatatt
      481 ttctgtcatt ctatggcctg acttttctta atggtttttt aattttaaaa ataagtctta
      541 atattcatgc aatctaatta acaatctttt ctttgtggtt aggactttga gtcataagaa
      601 atttttctct acactgaagt catgatggca tgcttctata ttattttcta aaagatttaa
      661 agttttgcct tctccattta gacttataat tcactggaat ttttttgtgt gtatggtatg
      721 acatatgggt tcccttttat tttttacata taaatatatt tccctgtttt tctaaaaaag
      781 aaaaagatca tcattttccc attgtaaaat gccatatttt tttcataggt cacttacata
      841 tatcaatggg tctgtttctg agctctactc tattttatca gcctcactgt ctatccccac
      901 acatctcatg ctttgctcta aatcttgata tttagtggaa cattctttcc cattttgttc
      961 tacaagaata tttttgttat tgtcttttgg gcttctatat acattttaga atgaggttgg
     1021 caagttgggg atcgtaccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1081 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1141 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1201 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1261 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     1321 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     1381 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
     1441 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     1501 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     1561 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     1621 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     1681 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     1741 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggctgtc
     1801 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1861 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1921 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttggatccac
     1981 tcgagttagc catggatgta ttcatgaaag gactttcaaa ggccaaggag ggagttgtgg
     2041 ctgctgctga gaaaaccaaa cagggtgtgg cagaagcagc aggaaagaca aaagagggtg
     2101 ttctctatgt aggctccaaa accaaggagg gagtggtgca tggtgtgaca acagtggctg
     2161 agaagaccaa agagcaagtg acaaatgttg gaggagcagt ggtgacgggt gtgacagcag
     2221 tagcccagaa gacagtggag ggagcaggga gcattgcagc agccactggc tttgtcaaaa
     2281 aggaccagtt gggcaagaat gaagagggag ccccacagga aggaattctg gaagatatgc
     2341 ctgtggatcc tgacaatgag gcttatgaaa tgccttctga ggaagggtat caagactacg
     2401 aacctgaagc cagatcttat ccgtatgatg ttcctgatta tgcttgatag taagcttatc
     2461 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2521 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2581 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2641 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2701 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2761 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2821 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     2881 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2941 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3001 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3061 cgtcgactcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3241 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3301 gctctatggc ttctgaggcg gaaagaacca gctggggctc gactagagca tggctacgta
     3361 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
     3421 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     3481 ccgggcggcc tcagtgagcg agcgagcgcg cagagctttt tgcaaaagcc taggcctcca
     3541 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     3601 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     3661 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     3721 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     3781 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     3841 tgacacacat tccacagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4501 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5881 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     5941 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6001 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     6061 atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc cttatgcgac
     6121 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     6181 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
     6241 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
     6301 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     6361 cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc
     6421 gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg
     6481 gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc
     6541 ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
     6601 atacgattta ggtgacacta tagaatacac ggaattaatt c
//
