LOCUS       sequence-49396-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry in cells lacking Cre (Cre-Off).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-DO-mCherry-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(50..83)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(134..167)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(460..479)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     729..747
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aaattttgta atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta
       61 tagcatacat tatacgaagt tattctttgc accattctaa agaataacag tgataatttc
      121 tgggttaagg caaataactt cgtataggat actttatacg aagttatgct agcgcggccg
      181 cttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact
      241 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt
      301 aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt
      361 agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct
      421 cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc
      481 cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca
      541 gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc
      601 acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca
      661 cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg
      721 ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgta
//
