LOCUS       sequence-49399-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, 
            expresses EGFP in Cre positive cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(26..59)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(110..143)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      165..174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(304..322)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     572..591
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     766..789
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cggatcctct agagtcgact ccggaataac ttcgtatagg atactttata cgaagttatg
       61 cagaatggta gctggattgt agctgctatt agcaatatga aacctcttaa taacttcgta
      121 tagcatacat tatacgaagt tatggcgcgc cttatctaga tttagccacc atggtgagca
      181 agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
      241 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      301 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
      361 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
      421 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      481 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
      541 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
      601 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
      661 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
      721 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
      781 cccacaacga ggactacacc atcgtggaac agtacgagcg ctcc
//
