LOCUS       sequence-49402-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Wiener R, Zhang X, Wang T, Wolberger C
  TITLE     The mechanism of OTUB1-mediated inhibition of ubiquitination.
  JOURNAL   Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911.
  PUBMED    22367539
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-02-22- 22"; volume: "483"; issue: "7391"; 
            pages: "618-22"
COMMENT     Sequence Label: ceOtub1
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 catcaccatc accatcacga ttacgatatc ccaacgaccg aaaacctgta ttttcagggc
       61 gccatggcca atgaacccca aaaatctgat gataacggtc aggctgcgga ggctgtggtg
      121 actgatgacg aaatagtttt gcaggaccaa cagttgaaga caattgaaga tgagcaaaaa
      181 tctgttccac ttgttgccac tttagcacca ttctctattc tttgtgctga atacgacaac
      241 gaaacatcgg ctgccttcct ttctaaagcg actgagctta gtgaagtgta tggagagatc
      301 cgctacattc gtggagacgg aaactgcttc tatcgtgcaa ttctcgttgg acttattgag
      361 ataatgctga aagatcgagc cagactcgaa aaattcattg cgagtagcag ggattggact
      421 cggacacttg ttgaacttgg atttccggat tggacctgca cagatttctg tgacttcttt
      481 atcgaattcc ttgagaaaat ccacagtgga gtgcataccg aggaagctgt ttacacgatt
      541 ttgaatgatg atggctctgc taattatatt ctcatgttct tccgtctcat aacttcagca
      601 tttttgaaac aaaactcaga agaatatgct ccattcattg atgagggaat gacagtggct
      661 caatattgcg aacaggaaat cgagccaatg tggaaagatg ccgatcatct ggcaattaat
      721 tctttgatca aagccgccgg cacaagagtt cgcatcgaat acatggaccg tactgctgct
      781 ccgaacggtg gttggcatta cgacattcca tctgacgatc aacaaattgc tccggagatc
      841 acacttctct atcgtccagg ccactacgat gtcatttaca aaaaggattc gactgaagcc
      901 tctgaaattg agaattgagg atcccggaat tcgaaaggcc ctacgtcga
//
